2011 Fiscal Year Final Research Report
Mechanism of brain tumor development by neuronal differentiation fail
Project/Area Number |
22790347
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Human pathology
|
Research Institution | Iwate Medical University |
Principal Investigator |
|
Project Period (FY) |
2010 – 2011
|
Keywords | 分子病理 |
Research Abstract |
(1) The expression ofβIII-tubulin is generally restricted to neurons, but its mRNA is often expressed at a low level in non-neuronal cells. Here, we present evidence thatβIII-tubulin expression occurs in a cell cycle-dependent manner. Both mRNA and protein ofβIII-tubulin accumulated around the G2/M stage of the cell cycle. Furthermore, the cell cycle-dependent expression ofβIII-tubulin was mediated by the neural-restrictive silencing factor NRSF/REST through its binding to the NRSE/RE-1 element that is present in the first intron of theβIII-tubulin gene. These results demonstrate a novel role ofβIII-tubulin in cell cycle progression in tumor cells. (2) Post-transcriptional acetylation-modification of cortactin(CTTN) via the nucleus accumbens-associated 1(NACC1)-histone deacetylase 6(HDAC6) deacetylation system were investigated. The acetylation status of CTTN modulated by the NACC1-HDAC6 deacetylation system induces acceleration of cell migration activity via an actin-dependent cellular process, possibly contributing to aggressive behavior(invasion/metastasis) of the tumor cells. (3) MicroRNAs(miRNAs) are small non-coding RNAs whose aberrations are involved in the initiation and progression of human cancers. We focused on one commonly downregulated miRNA(miR-211), and analyzed its relationship to the expression of preferentially expressed antigen of melanoma(PRAME) protein, which is a potential target of miR-211.These results suggest that downregulation of miR-211 may be partly involved in aberrant expression of the PRAME protein in melanoma cells.
|
Research Products
(7 results)
-
[Journal Article]2012
Author(s)
Ishikawa Y, Tsunoda K, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C
-
Journal Title
Int J Oncol.
Volume: (in press)
Peer Reviewed
-
[Journal Article]2012
Author(s)
Shibazaki M, Maesawa C, Akasaka K, Kasai S, Yasuhhira S, Kanno K, Nakayama I, Sugiyama T, Wakabayashi G, m Masuda T, Mori N
-
Journal Title
Int J Oncol.
Volume: 40
Pages: 695-702
Peer Reviewed
-
-
[Journal Article]2011
Author(s)
Sakurai E, Maesawa C, Shibazaki M, Yasuhira S, Oikawa H, Sato M, Tsunoda K, Ishikawa Y, Watanabe A, Takahashi K, Akasaka T, Masuda T
-
Journal Title
Int J Oncol.
Volume: 39
Pages: 665-672
Peer Reviewed
-
[Journal Article]2011
Author(s)
Tsunoda K, Oikawa H, Tada H, Tatemichi Y, Muraoka S, Miura S, Shibazaki M, Maeda F, Takahashi K, Akasaka T, Masuda T, Maesawa C
-
Journal Title
J Invest Dermatol.
Volume: 131
Pages: 1710-1719
Peer Reviewed
-
[Journal Article]2011
Author(s)
Ebina M, Shibazaki M, Kudo K, Kasai S, Kikuchi H
-
Journal Title
Biochem Biophys Acta.
Volume: 1809
Pages: 176-183
Peer Reviewed
-
[Presentation]2011
Author(s)
前沢千早、柴崎晶彦、安平進士、葛西秋宅
Organizer
日本分子生物学会総会
Place of Presentation
横浜
Year and Date
20110000