Analysis of cell adhesion molecules involved in progression of malignant mesothelioma

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790362
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: Hyogo Medical University
• Principal Investigator: SATO Ayuko 兵庫医科大学, 医学部, 講師 (20419823)
• Project Period (FY): 2010 – 2011
• Keywords: 悪性中皮腫 / 細胞接着分子 / CD146 / 胸水細胞診

Research Abstract

We evaluated the expression of cell adhesion molecules in malignant pleural mesothelioma, and found that CD146 was strongly expressed in mesothelioma cells but not in reactive mesothelial cells, in pleural effusion fluids. Using an orthotopic implantation model of malignant pleural mesothelioma, we showed that CD146 was expressed in diffusely growing thoracic tumors, but not in murine mesothelial cells. Our results suggest that CD146 is up-regulated upon malignant transformation of mesothelial cells, and might be involved in manifestation of cytopathological features of malignant mesothelioma cells. We propose that CD146 is an effective immunostaining marker enabling discrimination between malignant pleural mesothelioma and reactive mesothelium in effusion cytology, and useful for early diagnosis of malignant mesothelioma.

Research Products

• [Journal Article] Establishment of a cell line from a Japanese patient useful for generating an in vivo model of malignant pleural mesothelioma (2011)
  • Author(s): Sato A, Torii I, Tao LH, Song M, Kondo N, Yoshikawa Y, Hashimoto-Tamaoki T, Hasegawa S, Nakano T, and Tsujimura T.
  • Journal Title: Cancer Sci. Volume: 102(3) Pages: 648-655
  • DOI: DOI:10.1111/j.1349-7006.2010.01827.x
  • Peer Reviewed
• [Journal Article] Frequent deletion of 3p21.1 region carrying semaphorin 3G and aberrant expression of the genes participating in semaphorin signaling in the epithelioid type of malignantmesothelioma cells (2011)
  • Author(s): Yoshikawa Y, Sato A, Tsujimura T, Morinaga T, Fukuoka K, Yamada S, Murakami A, Kondo N, Matsumoto S, Okumura Y, Tanaka F, Hasegawa S, Hashimoto-Tamaoki T, and Nakano T.
  • Journal Title: Int J Oncol. Volume: 39(6) Pages: 1365-74
  • DOI: DOI:10.3892/ijo.2011.1158
  • Peer Reviewed
• [Journal Article] Immunocytochemistry of CD146 is useful to discriminate between malignant pleural mesothelioma and reactive mesothelium (2010)
  • Author(s): Sato A, Torii I, Okamura Y, Yamamoto T, Nishigami T, Kataoka TR, Song M, Hasegawa S, Nakano T, Kamei T, and Tsujimura T.
  • Journal Title: Mod. Pathol. Volume: 23 Pages: 1458-1466
  • DOI: DOI:10.1038/modpathol.2010.134
  • Peer Reviewed
• [Presentation] 悪性中皮腫の鑑別診断におけるCD146免疫染色の有用性 (2011)
  • Author(s): 佐藤鮎子, 鳥井郁子, 中野孝司, 長谷川誠紀, 亀井敏昭, 辻村亨
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20110428-30
• [Presentation] 悪性胸膜中皮腫の病理学的解析:病態モデルの作製と診断マーカーの開発(平成22年度日本病理学会近畿支部学術奨励賞公募部門受賞講演) (2011)
  • Author(s): 佐藤鮎子
  • Organizer: 第53回日本病理学会近畿支部学術集会
  • Place of Presentation: 兵庫医科大学
  • Year and Date: 2011-05-14
• [Presentation] 中皮腫診断と細胞診悪性胸膜中皮腫の早期診断への糸口中皮腫の細胞学的特徴を分子レベルで考える (2010)
  • Author(s): 辻村亨, 佐藤鮎子, 鳥井郁子, 亀井敏昭, 長谷川誠紀, 中野孝司
  • Organizer: (シンポジウム)第51回日本肺癌学会総会
  • Place of Presentation: 広島国際会議場・ANAクラウンプラザホテル広島
  • Year and Date: 20101103-04
• [Presentation] 体腔液細胞診での悪性中皮腫と反応性中皮の鑑別診断におけるCD146の有用性 (2010)
  • Author(s): 佐藤鮎子, 鳥井郁子, 片岡竜貴, 長谷川誠紀, 中野孝司, 辻村亨
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル大阪
  • Year and Date: 20100922-24
• [Presentation] 早期中皮腫の体腔液細胞診におけるp16INK4A遺伝子解析の有用性 (2010)
  • Author(s): 辻村亨, 佐藤鮎子, 鳥井郁子, 片岡竜貴, 長谷川誠紀, 中野孝司
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル大阪
  • Year and Date: 20100922-24
• [Presentation] Diagnositic significance of p16INK4A loss in molecular cytology of early malignant pleural mesothelioma (2010)
  • Author(s): Tsujimura T, Sato A, Torii I, Kataoka TR, Nishigami T, Yoshikawa Y, Hashimoto-Tamaoki T, Hasegawa S, and Nakano T.
  • Organizer: 10th International Conference of the International Mesothelioma InterestGroup
  • Place of Presentation: Kyoto
  • Year and Date: 20100831-0903
• [Presentation] Immunocytochemistry of CD146 is useful for discrimination between malignant pleural mesothelioma and reactive mesothelium in effusion cytology (2010)
  • Author(s): Sato A, Torii I, Okamura Y, Yamamoto T, Nishigami T, Kataoka TR, Hasegawa S, Nakano T, and Tsujimura T.
  • Organizer: 10th International Conference of the International Mesothelioma Interest Group
  • Place of Presentation: Kyoto
  • Year and Date: 20100831-0903
• [Presentation] Usefulness of gene diagnosis of the p16INK4A locus in molecular cytology of early malignant pleural mesothelioma (2010)
  • Author(s): Tsujimura T, Sato A, Torii I, Kataoka TR, Nishigami T, Yoshikawa Y, Hashimoto-Tamaoki T, Hasegawa S, and Nakano T.
  • Organizer: 17th International Congress of Cytology
  • Place of Presentation: Edinburgh
  • Year and Date: 20100516-20
• [Presentation] 悪性胸膜中皮腫モデルマウスの作製と病理学的解析 (2010)
  • Author(s): 佐藤鮎子, 鳥井郁子, 西上隆之, 片岡竜貴, 中野孝司, 長谷川誠紀, 辻村亨
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 20100427-29
• [Book] 中皮腫の初期病変.癌診療指針のための病理診断プラクティス肺癌. 4章胸膜腫瘍疾患の概要と鑑別診断 (2011)
  • Author(s): 辻村亨、鳥井郁子、佐藤鮎子
  • Total Pages: 247-258
  • Publisher: 中山書店