Functional analysis of Trem-like 4 in mice

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Osaka University (2011); The Institute of Physical and Chemical Research (2010)
• Principal Investigator: HEMMI Hiroaki 大阪大学, 免疫学フロンティア研究センター, 寄附研究部門助教 (20451924)
• Project Period (FY): 2010 – 2011
• Keywords: 樹状細胞 / マクロファージ / イムノグロブリンスーパーファミリー

Research Abstract

Triggering Receptor Expressed on Myeloid cells(Trem) family is characterized by an immunoglobulin-like domain at extracellular domain and short cytoplasmic tail. In this study, we aimed to clarify physiological functions of a Trem family member, Trem-like 4(Treml4). We could found a candidate molecule of Treml4 ligand. In addition, we analyzed Treml4-deficent mice. In Treml4-deficient mice, uptake of dying cells and cross-presentation of cell-associated antigens in vivo was similarly observed to that in littermate control mice, suggesting that Treml4 is not involved in these responses. We also found that an engineered anti-Treml4 antibody conjugated with antigens can efficiently induce T cell responses.

Research Products

• [Journal Article] Treml4, an Ig Superfamily Member, Mediates Presentation of Several Antigens to T Cells In Vivo, Including Protective Immunity to HER2 Protein (2012)
  • Author(s): Hiroaki Hemmi, Neeha Zaidi, Bei Wang, Ines Matos, Christopher Fiorese, Ashira Lubkin, Lori Zbytnuik, Koji Suda, Kenneth Zhang, Masaki Noda, Tsuneyasu Kaisho, Ralph M. Steinman, and Juliana Idoyaga
  • Journal Title: J Immunol Volume: 188 Pages: 1147-1155
  • DOI: DOI:10.4049/jimmunol.1102541
  • Peer Reviewed
• [Journal Article] CD8陽性樹状細胞サブセットの動的制御機構の解析 (2012)
  • Author(s): 邊見弘明, 山崎千尋, 星野克明, 改正恒康
  • Journal Title: Cytometry Research Volume: 22 Pages: 31-35
  • URL: http://www.cytometry.jp/scientist/journals/%E3%83%90%E3%83%83%E3%82%AF%E3%83%8A%E3%83%B3%E3%83%90%E3%83%BC
  • Peer Reviewed
• [Journal Article] Conservation of a chemokine system, XCR1 and its ligand, XCL1, between human and mice (2010)
  • Author(s): Chihiro Yamazaki, Rie Miyamoto, Katsuaki Hoshino, Yuri Fukuda, Izumi Sasaki, Masuyoshi Saito, Hironori Ishiguchi, Takahiro Yano, Takahiro Sugiyama, Hiroaki Hemmi, Takashi Tanaka, Eri Hamada, Takeshi Hirashima, Ryuichi Amakawa, Shirou Fukuhara, Shosaku Nomura, Tomoki Ito, Tsuneyasu Kaisho
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Pages: 756-761
  • DOI: DOI:10.1016/j.bbrc.2010.06.029
  • Peer Reviewed
• [Presentation] ケモカイン受容体Xcr1発現樹状細胞のin vivoにおける機能的意義 (2011)
  • Author(s): 山崎千尋, 邊見弘明, 宮本理恵, 佐々木泉, 伊藤量基, 星野克明, 改正恒康
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2011-11-28
• [Presentation] CD8陽性樹状細胞の動的制御機構の解析 (2011)
  • Author(s): 邊見弘明, 山崎千尋, 星野克明, 改正恒康
  • Organizer: 第21回日本サイトメトリー学会学術集会
  • Place of Presentation: 京都市国際会館
  • Year and Date: 2011-06-26