2011 Fiscal Year Final Research Report
Analysis in activation of hepatic stem/progenitor cells and development of application to hepatic tissue engineering
| Project/Area Number |
22790385
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 細胞・組織 / 再生医学 / 肝幹・前駆細胞 / 生体組織工学 / 細胞移植 / 分化 |
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| Research Abstract |
Oval cells and small hepatocytes(SHs) are known as hepatic stem and progenitor cells, and Thy1 and CD44 are used as a marker, respectively. Although oval cells are believed to differentiate into mature hepatocytes(MHs) through SHs, the details of their differentiation process are not well understood. In the present experiment we used Thy1^+ oval and CD44^+cells isolated from D-galactosamine-treated rat livers. (1) EGF, basic FGF, or HGF could trigger the hepatic differentiation of sorted Thy1^+ cells to form epithelial cell colonies. (2) Thy1^+oval and CD44^+cells are able to differentiate into hepatocytes, the degree of hepatic maturation of the induced hepatocytes may not be equal to that of normal resident ones. (3) Thy1^+, CD44^+cells and MHs were transplanted into livers treated with retrorsine following partial hepatectomy. Both stem and progenitor cells could differentiate into hepatocytes in host livers. Repopulation efficiency and the survival period of Thy1^+, CD44^+ cells in the long term was very low. The short life of the cells may be due to their cellular senescence.
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[Presentation] 肝硬変ラットモデルにおける肝細胞移植の有用性についての検討2010
Author(s)
中村幸雄, 水口徹, 柴田稔人, 谷水直樹, 大栄秀和, 市戸義久, 川本雅樹, 目黒誠, 古畑智久, 木村康利, 信岡隆幸, 三高俊広, 平田公一
Organizer
第14回肝臓学会大会
Place of Presentation
横浜
Year and Date
20101013-14
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