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2011 Fiscal Year Final Research Report

The host RNA uptake related gene in Schistosoma japonicum

Research Project

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Project/Area Number 22790393
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Parasitology (including Sanitary zoology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUMAGAI Takashi  東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40369054)

Co-Investigator(Renkei-kenkyūsha) OHTA Nobuo  東京医科歯科大学, 医歯学総合研究科, 教授 (10143611)
Project Period (FY) 2010 – 2011
Keywords蠕虫 / RNAi / 住血吸虫 / sid-1
Research Abstract

Schistosomes induce the RNA interference(RNAi) using long dsRNA in soaking method, such as C. elegans. It is previously reported that schistosomes incorporate the dsRNA, including host derived RNA. To understand the relationships between RNA uptake and parasitism, sid-1 gene, related to RNAi machinery, was investigated. The mRNA of sid-1 was increased in the larval stage after the invasion to the host, and was regulated by the induction of long dsRNA. However, the schistosomes knocked down with sid-1 siRNA, were not effects to RNAi machinery, dsRNA uptake, and parasitism in the host. These mean that schistosomes may have the distinct RNAi machinery.

  • Research Products

    (19 results)

All 2012 2011 2010

All Journal Article (10 results) (of which Peer Reviewed: 6 results) Presentation (9 results)

  • [Journal Article] Phylogenetic relationships ofrat lungworm, Angiostrongylus cantonensis, isolated from different geographical regions revealed widespread multiple lineages2012

    • Author(s)
      Tokiwa T, Harunari T, Tanikawa T, Komatsu N, Koizumi N, Tung KC, Suzuki J, Kadosaka T, Takada N, Kumagai T, Akao N, Ohta N
    • Journal Title

      Parasitol Int

      Volume: (in press)

  • [Journal Article] Loop-mediated isothermal amplification(LAMP): early detection of Toxoplasma gondii infection in mice2012

    • Author(s)
      Kong QM, Lu SH, Tong QB, Lou D, Chen R, Zheng B, Kumagai T, Wen LY, Ohta N, Zhou XN.
    • Journal Title

      Parasit Vectors

      Volume: 3 Pages: 5 : 2

  • [Journal Article] Interleukin-4(IL-4) and IL-13 suppress excessive neutrophil infiltration and hepatocyte damage during acute murine schistosomiasis japonica2012

    • Author(s)
      Seki T, Kumagai T, Kwansa-Bentum B, Furushima-Shimogawara R, Anyan WK, Miyazawa Y, Iwakura Y, Ohta N
    • Journal Title

      Infect Immun

      Volume: 80 Pages: 159-68

  • [Journal Article] Cerebral Schistosomiasis Due to Schistosoma haematobium Confirmed by PCR Analysis of Brain Specimen2011

    • Author(s)
      Imai K, Koibuchi T, Kumagai T, Maeda T, Osada Y, Ohta N, Koga M, Nakamura H, Miura T, Iwamoto A, Fujii T
    • Journal Title

      J Clin Microbiol

      Volume: 49 Pages: 3703-6

  • [Journal Article] Plasmodium falciparum isolates from southern Ghana exhibit polymorphisms in the SERCA-type PfATPase6 though sensitive to artesunate in vitro2011

    • Author(s)
      Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Osei JH, Asahi H, Ofori MF, Akao N, Wilson MD, Boakye DA, Ohta N.
    • Journal Title

      Malar J

      Volume: 11 Pages: 10 : 187

    • Peer Reviewed
  • [Journal Article] Administrative practices of health professionals and use of artesunate-amodiaquine by community members for treating uncomplicated malaria in southern Ghana : implications for artemisinin-based combination therapy deployment2011

    • Author(s)
      Kwansa-Bentum B, Ayi I, Suzuki T, Otchere J, Kumagai T, Anyan WK, Asahi H, Akao N, Wilson MD, Boakye DA, Ohta N
    • Journal Title

      Trop Med Int Health

      Volume: 10 Pages: 134-139

    • Peer Reviewed
  • [Journal Article] Schistosomicidal and antifecundity effects of oral treatment of synthetic endoperoxide N-892011

    • Author(s)
      Taniguchi T, Kumagai T, Shimogawara R, Ichinose S, Hiramoto A, Sato A, Morita M, Nojima M, Kim HS, Wataya Y, Ohta N.
    • Journal Title

      Parasitol Int

      Volume: 60 Pages: 231-6

    • Peer Reviewed
  • [Journal Article] Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification(LAMP) assay2010

    • Author(s)
      Kumagai T, Furushima-Shimogawara R, Ohmae H, Wang TP, Lu S, Chen R, Wen L, Ohta N.
    • Journal Title

      Am J Trop Med Hyg

      Volume: 83 Pages: 542-8

    • Peer Reviewed
  • [Journal Article] Loop-mediated isothermal amplification assay for detection and discrimination of Toxocara canis and T. cati eggs directly from sand samples2010

    • Author(s)
      Macuhova K, Kumagai T, Akao N, Ohta N.
    • Journal Title

      J. Parasitol.

      Volume: 96 Pages: 1224-7

    • Peer Reviewed
  • [Journal Article] Schistosome eggs have a direct role in the induction of basophils capable of a high level of IL-4 production : Comparative study of single-and bisexual infection of Schistosoma mansoni in vivo2010

    • Author(s)
      Anyan KW, Kumagai T, Shimogawara FR, Seki T, Akao N, Obata K, Bentum KB, Bosompen MK, Boakye AD, Wilson DM, Karasuyama H, Ohta N
    • Journal Title

      Tropical Medicine and Health

      Volume: 38 Pages: 13-22

    • Peer Reviewed
  • [Presentation] Rapid mRNA degradation by soaking method of antisense RNA, but not dsRNA in Schistosoma japonicum2012

    • Author(s)
      Kumagai T, et al
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      20120900
  • [Presentation] 日本住血吸虫成虫より分泌されるエクソソーム様小胞の免疫抑制作用2012

    • Author(s)
      熊谷貴ら
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      西宮
    • Year and Date
      20120300
  • [Presentation] 日本住血吸虫感染貝モニタリングを目指したLAMP法の適用2011

    • Author(s)
      熊谷貴ら
    • Organizer
      第52回日本熱帯医学会大会・第26回日本国際保険医療学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      20111100
  • [Presentation] The application of PCR and loop-mediated isothermal amplification(LAMP) methods to the field surveillance for snail infections of Schistosoma japonicum2011

    • Author(s)
      Kumagai, et al
    • Organizer
      The 59th ASTMH Annual Meeting
    • Place of Presentation
      Atlanta
    • Year and Date
      20111100
  • [Presentation] Characterization of systemic RNAi deficiency-1 from Schistosoma japonicum and the mechanisms of dsRNA incorporation2011

    • Author(s)
      Kumagai, et al
    • Organizer
      The 12th International Congress of Parasitology
    • Place of Presentation
      Melbourne
    • Year and Date
      20110800
  • [Presentation] 日本住血吸虫でみられたアンチセンスRNAによるRNA干渉2011

    • Author(s)
      熊谷貴ら
    • Organizer
      第22回日本生体防御学会学術総会
    • Place of Presentation
      那覇
    • Year and Date
      20110600
  • [Presentation] 日本住血吸虫RNAi関連分子SID-1のmRNAヴァリアントの機2011

    • Author(s)
      熊谷貴ら
    • Organizer
      第80回日本寄生虫学会大会
    • Place of Presentation
      東京
    • Year and Date
      20110300
  • [Presentation] 日本住血吸虫のSID-1とスカベンジャー受容体はそれぞれ異なった場所でRNA干渉に関わる2010

    • Author(s)
      熊谷貴ら
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      20101200
  • [Presentation] イメージング解析による日本住血吸虫の宿主RNA取り込み機構の解析2010

    • Author(s)
      熊谷貴ら
    • Organizer
      第79回日本寄生虫学会大会
    • Place of Presentation
      旭川
    • Year and Date
      20100500

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Published: 2013-07-31  

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