Studies on apoptotic signaling in macrophages following mycobacterial infection

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790407
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Shimane University
• Principal Investigator: TATANO Yutaka 島根大学, 医学部, 助教 (70432614)
• Project Period (FY): 2010 – 2011
• Keywords: マクロファージ / アポトーシス / 細菌 / シグナル伝達 / 殺菌エフェクター

Research Abstract

In this study, we examined the correlation between apoptotic signaling pathways and macrophage activities against Mycobaccterium smegmatis(SM) infection. Treatment with 5 mM ATP or 50μM 1-(3, 4-Dichlorobenzyl)-1H-indole-2, 3-dion : Apoptosis activatorII(AAII) induced the apoptosis of macrophages and decreased intramacrophage survival of SM. AAII-induced but not ATP-induced apoptosis recovers in macrophages treated with caspase 3 inhibitor. These results suggest that apoptosis induced by AAIImay be concomitant with the up-regulation of macrophage anti-mycobacterial activities, and this affects a signal transduction involved in caspase 3-mediated apoptosis.

Research Products

• [Journal Article] Characteristics of Suppressor Macrophages Induced by Mycobacterial and Protozoal Infections in Relation to Alternatively Activated M2 Macrophages (2012)
  • Author(s): Tomioka H, Tatano Y, Maw WW, Sano C, Kanehiro Y, Shimizu T
  • Journal Title: Clin Dev Immunol Pages: 19
  • DOI: doi:10.1155/2012/635451
• [Journal Article] Correlation between variable-number tandem-repeat-based genotypes and drug susceptibility in Mycobacterium avium isolates (2012)
  • Author(s): Tatano Y, Sano C, Yasumoto K, Shimizu T, Sato K, Nishimori K, Matsumoto T, Yano S, Takeyama H, Tomioka H
  • Journal Title: Eur J Clin Microbiol Infect Dis Volume: 31 Pages: 445-454
  • DOI: DOI:10.1007/s10096-011-1326-7
  • Peer Reviewed
• [Journal Article] Development of new antituberculous drugs based on bacterial virulence factors interfering with host cytokine networks (2011)
  • Author(s): Tomioka H, Tatano Y, Sano C, Shimizu T
  • Journal Title: J Infect Chemother Volume: 3 Pages: 302-317
  • DOI: DOI:10.1007/s10156-010-0177-y
• [Journal Article] Comparative study for the virulence of Mycobacterium avium isolates from patients with nodular-bronchiectasis-and cavitarytype diseases (2010)
  • Author(s): Tatano Y, Yasumoto K, Shimizu T, Sano C, Sato K, Yano S, Takeyama H, Tomioka H
  • Journal Title: Eur J Clin Microbiol Infect Dis Volume: 29 Pages: 801-806
  • DOI: DOI:10.1007/s10096-010-0930-2
  • Peer Reviewed
• [Presentation] Association between variable-number tandem-repeat-based genotypes and drug susceptibility in Mycobacterium avium isolates (2011)
  • Author(s): Yutaka Tatano, Chiaki Sano, Toshiaki Shimizu, Haruaki Tomioka
  • Organizer: International Union of Microbiological Societies 2011 Congress(IUMS 2011)
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan
  • Year and Date: 2011-09-10
• [Presentation] Correlation between variable-number tandem-repeat-based genotypes and drug susceptibility in Mycobacterium avium isolates (2011)
  • Author(s): Yutaka Tatano, Chiaki Sano, Toshiaki Shimizu, Haruaki Tomioka
  • Organizer: The 8th International Symposium on Antimicrobial Agents and Resistance(ISAAR 2011)
  • Place of Presentation: COEX, Seoul, Korea
  • Year and Date: 2011-04-07
• [Presentation] Properties of immunosuppressive macrophages generated by Mycobacterium avium complex infection induced in bcgs and bcgr genotype mice (2010)
  • Author(s): Yutaka Tatano, Chiaki Sano, Toshiaki Shimizu, and Haruaki Tomioka
  • Organizer: Society for general microbiology(SGM) Autumn Conference-University of Nottingham
  • Place of Presentation: Nottingham, UK
  • Year and Date: 20100906-09