2012 Fiscal Year Final Research Report
Study on the molecular mechanisms of host adaptation of Leptospira interrogans
| Project/Area Number |
22790422
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
KOIZUMI Nobuo 国立感染症研究所, 細菌第一部, 主任研究官 (10333361)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 病原性 / 人獣共通感染症 / レプトスピラ / スピロヘータ |
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| Research Abstract |
To identify leptospiral proteins which function in cell attachment, we employed recombinant T7 phage and Escherichia coli which expresses leptospiral proteins fused with 10B capsid protein and AIDA transporter protein on its cell surface, respectively. We screened recombinant phages and E coli cells which bound to cultured cells but failed to obtain specific clones. We established a method of mariner transposon mediated mutagenesis in pathogenic and non-pathogenic Leptospira spp by conjugational transfer with E coli.
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