Molecular characterization of novel isoniazid resistance mechanism in Mycobacterium tuberculosis

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790423
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Research Institute, International Medical Center of Japan
• Principal Investigator: ANDO Hiroki 独立行政法人国立国際医療研究センター, 感染症制御研究部, 上級研究員 (70462786)
• Project Period (FY): 2010 – 2011
• Keywords: 結核 / 薬剤耐性 / イソニアジド

Research Abstract

Novel isoniazid (INH) resistance mutations were identified and characterized in Mycobacterium tuberculosis (Mtb). I collected INH-resistant Mtb clinical isolates and found that many of them have no mutations in known INH resistance genes. I identified several novel mutations in these isolates, and analyzed their roles in INH resistance.

Research Products

• [Journal Article] Downregulation of katG expression is associated with isoniazid resistance in Mycobacterium tuberculosis. (2011)
  • Author(s): Ando H, Kitao T, Miyoshi-Akiyama T, Kato S, Mori T, Kirikae T
  • Journal Title: Mol Microbiol Volume: 79(6) Pages: 1615-1628
  • DOI: doi:10.1111/j.1365-2958.2011.07547.x
  • Peer Reviewed
• [Journal Article] Evaluation of a line probe assay for rapid detection of gyrA mutations associated with fluoroquinolone resistance in multidrug-resistant Mycobacterium tuberculosis. (2011)
  • Author(s): Ando H, Mitarai S, Kondo Y, Suetake T, Kato S, Mori T, Kirikae T
  • Journal Title: J Med Microbiol Volume: 60(Pt 2) Pages: 184-188
  • DOI: doi:10.1099/jmm.0.024729-0.
  • Peer Reviewed
• [Journal Article] Pyrazinamide resistance in multidrug-resistant Mycobacterium tuberculosis isolates in Japan. (2010)
  • Author(s): Ando H, Mitarai S, Kondo Y, Suetake T, Sekiguchi JI, Kato S, Mori T, Kirikae T
  • Journal Title: Clin Microbiol Infect Volume: 16(8) Pages: 1164-1168
  • DOI: doi:10.1111/j.1469-0691.2009.03078.x
  • Peer Reviewed
• [Journal Article] Identification of katG mutations associated with high-level isoniazid resistance in Mycobacterium tuberculosis. (2010)
  • Author(s): Ando H, Kondo Y, Suetake T, Toyota E, Kato S, Mori T, Kirikae T
  • Journal Title: Antimicrob Agents Chemother Volume: 54(5) Pages: 1793-1799
  • DOI: doi:10.1128/AAC.01691-09.
  • Peer Reviewed
• [Presentation] Downregulation of katG expression is associated with isoniazid resistance in Mycobacterium tuberculosis. (2011)
  • Author(s): Ando H, Kitao T, Miyoshi-Akiyama T, Kato S, Mori T, Kirikae T
  • Organizer: IUMS 2011 Congress
  • Place of Presentation: Japan(Poster No. P-BA28-23)
  • Year and Date: 20110900
• [Presentation] Mutations in the furA-katG intergenic region decrease katG expression and confer isoniazid resistance in Mycobacterium tuberculosis. (2011)
  • Author(s): Ando H, Kitao T, Miyoshi-Akiyama T, Kato S, Mori T, Kirikae T
  • Organizer: 4^<th> Congress of European Microbiologists : FEMS 2011
  • Place of Presentation: Switzerland(Poster No. 67)
  • Year and Date: 20110600
• [Presentation] 結核菌のfabG1 遺伝子内サイレント変異によるイソニアジド耐性機序の解明 (2010)
  • Author(s): 安藤弘樹, 加藤誠也, 森亨, 切替照雄
  • Organizer: 第33回日本分子生物学会年会・第83 回日本生化学会大会合同大会
  • Place of Presentation: 兵庫県神戸市(ポスター番号 1P-1072)
  • Year and Date: 20101200
• [Presentation] Down-regulation of katG expression is associated with isoniazid resistance in Mycobacterium tuberculosis. (2010)
  • Author(s): Ando H, Kato S, Mori T, Kirikae T
  • Organizer: 110^<th> General Meeting of the American Society for Microbiology
  • Place of Presentation: USA(Poster No. U-3153)
  • Year and Date: 20100500