2011 Fiscal Year Final Research Report
M13 bacteriophage-based vaccine design for prion.
| Project/Area Number |
22790435
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | バクテリオファージ / ワクチン / 自然免疫 / プリオン / 抗体 / ファージディスプレイ |
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| Research Abstract |
Phage display library is powerful tool to develop various recombinant peptides or human antibodies applicable for revolutionary therapeutics. In addition to this feature, peptide-displaying phage clones induce mimotope-specific antibody responses in mice. In this study, we have established a human IgG1 antibody specific to s-form prion protein(PrP) but not to.-form PrP, PRB7 IgG. Employing a peptide-displaying phage library, the mimotope was determined to be at# 128-132 of human PrP. A single injection of peptide-displaying phage stimulate innate immune response and elicited a strong IgG response in mice, suggests a possible application of peptide-displaying phage clones as vaccine carriers for induction of therapeutic antibodies.
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[Journal Article] Direct evidence of generation and accumulation of β-sheet-rich prion protein in ScN2a cells de novo illuminated by human IgG1 antibody recognizing β-form but not α-form of prion protein2012
Author(s)
Toshiya Kubota, Yuta Hamazoe, Shuhei Hashiguchi, Daisuke Ishibashi, Kazuyuki Akasaka, Noriyuki Nishida, Shigeru Katamine, Suehiro Sakaguchi, Ryota Kuroki, Toshihiro Nakashima, Kazuhisa Sugimura.
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Journal Title
The Journal of biological chemistry
Volume: 287巻
Pages: 14023-14039
DOI
Peer Reviewed
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