2011 Fiscal Year Final Research Report
Development of the methods for site-specific integration of LV vector with novel ZFP-LEDGF fusion protein
| Project/Area Number |
22790436
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Yokohama City University |
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Principal Investigator |
KONDO Asami (井野 麻美) 横浜市立大学, 医学部, 助教 (30453046)
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| Research Collaborator |
RYO Akihide 横浜市立大学, 医学研究科, 教授 (20363814)
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| Project Period (FY) |
2010 – 2011
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| Keywords | レンチウイルスベクター / 配列特異的挿入 / 遺伝子ノックアウト |
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| Research Abstract |
Although lentiviral(LV) vector genomic integration step is essential for gene expression, mutations are found in host genome accompanied with this step. This aim of project is to develop of the methods for site-specific integration of LV vector. I fused the essential protein for integration of LV vector and DNA binding domain which was designed to bind sequence specific DNA. I could not find the sequence-specific integration of LV vector with these fusion proteins.
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