2011 Fiscal Year Final Research Report
Functional analysis of CM2 in influenza C virus replication by using the mutants of the posttranslational modification sites
| Project/Area Number |
22790439
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
OKUWA Takako 金沢医科大学, 医学部, 助教 (20460347)
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| Project Period (FY) |
2010 – 2011
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| Keywords | C型インフルエンザウイルス / CM2蛋白 / 翻訳後修飾 |
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| Research Abstract |
To investigate the role(s) of CM2 in the virus replication, we generated and analysed recombinant influenza C viruses lacking the posttranslational modification sites. As a result, the recombinant viruses, in which the glycosylation site or three cysteine residues involved in oligomerization of CM2 were mutated to alanine, grew less efficiently than the wild-type virus. Analyses using virus-like particles revealed that glycosylation of CM2 is involved in the genome packaging and uncoating processes.
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