2011 Fiscal Year Final Research Report
The molecular basis of substrate recognition by KSHV MIR
Project/Area Number |
22790442
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Virology
|
Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
KAJIKAWA Mizuho 独立行政法人理化学研究所, 感染免疫応答研究チーム, 基礎科学特別研究員 (00464389)
|
Project Period (FY) |
2010 – 2011
|
Keywords | ウイルス / 免疫回避 / 免疫受容体 / 膜タンパク質 / ユビキチンリガーゼ |
Research Abstract |
MIR is a membrane associated ubiquitin ligase family encoded in KSHV. MIR is an immune evasion molecule because it induces the lysosomal degradation of immunoreceptors by ubiquitination. In this study, I found that both transmembrane and extracellular region of MIR play a crucial role in immunoreceptor recognition. To clarify the molecular basis of this recognition, I developed a recombinant MIR expression system and a purification/solubilization method for NMR analysis.
|
Research Products
(3 results)