2013 Fiscal Year Final Research Report
Measles virus infection of epitherial cells
| Project/Area Number |
22790445
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
TAHARA Maino 国立感染症研究所, ウイルス第三部, 主任研究官 (10572109)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 麻疹ウイルス |
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| Research Abstract |
Monoclonal antibodies (MAbs) recognizing distinct epitopes on the H protein were characterized using recombinant MVs. We identified the location of five epitopes on the H protein structure. Our data in the present study demonstrated that the H protein of MV possesses at least two conserved effective neutralizing epitopes. One, which is a previously recognized epitope, is located near the receptor-binding site (RBS), whereas the other epitope is located at the position distant from the RBS. MAb that recognizes this epitope interfered with the hemagglutinin-fusion (H-F) interaction. This epitope was suggested to play a key role for formation of a higher order of an H-F protein oligomeric structure. Our data also identified one nonconserved effective neutralizing epitope. This epitope has been masked by an N-linked sugar modification in some genotype MV strains.
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Research Products
(17 results)
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[Journal Article] The receptor-binding site of the measles virus hemagglutinin protein itself constitutes a conserved neutralizing epitope2013
Author(s)
Tahara M, Ohno S, Sakai K, Ito Y, Fukuhara H, Komase K, Brindley MA, Rota PA, Plemper RK, Maenaka K, Takeda M.
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Journal Title
J Virol.
Volume: 87巻
Pages: 3583-3586
DOI
Peer Reviewed
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[Journal Article] Functional and structural characterization of neutralizing epitopes of measles virus hemagglutinin protein2013
Author(s)
Tahara M, Ito Y, Brindley MA, Ma X, He J, Xu S, Fukuhara H, Sakai K, Komase K, Rota PA, Plemper RK, Maenaka K, Takeda M.
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Journal Title
J Virol.
Volume: 87巻
Pages: 666-675
DOI
Peer Reviewed
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[Presentation] 麻疹ウイルス単一血清型決定の分子基盤2012
Author(s)
田原舞乃, Melinda A. Brindley, 福原秀雄, 酒井宏治, 大野真治, 駒瀬勝啓, Paul A. Rota , Richard K. Plemper, 前仲勝実, 竹田誠
Organizer
第60回日本ウイルス学会学術集会
Place of Presentation
大阪
Year and Date
20121100
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[Presentation] Virological, Structural and Molecular Bases for the Unchanged Antigenicity of Measles Virus2012
Author(s)
Tahara M, Ito Y, Brindley MA, Ma XM, He JL, Xu ST, Fukuhara H, Sakai K, Ohno S, Komase K, Rota PA, Plemper RK, Maenaka K, and Takeda M.
Organizer
Osong Symposium on Infectious Disease
Place of Presentation
韓国
Year and Date
20120925-26
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[Presentation] A structural and biochemical basis for the single serotype nature of MV2012
Author(s)
Tahara M, Ito Y, Brindley MA, Ma XM, He JL, Xu ST, Fukuhara H, Sakai K, Ohno S, Komase K, Rota PA, Plemper RK, Maenaka K, and Takeda M
Organizer
The 11th Awaji International Forum on Infection and Immunity
Place of Presentation
Hyogo, Japan
Year and Date
20120911-14
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