Visualization and functional analysis of Mincle expressi on cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790471
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Kyushu University
• Principal Investigator: MIYAKE Yasunobu 九州大学, 生体防御医学研究所分子免疫学分野, 助教 (10392143)
• Project Period (FY): 2010 – 2011
• Keywords: 自然免疫 / C型レクチン / Mincle / 肉芽腫

Research Abstract

I have generated GFP knock-in mice for visualization of Mincle-expressing cells invivo. In steady-state condition, GFP positive cells were not detected in peripheral blood, bone marrow, spleen, lymph node and lung. On the other hand, abundant number ofGFP positive cells were accumulated in the lung granulomas induced by mycobacterialglycolipid, TDM administration. Most of these GFP positive cells were neutrophils(CD11b positive and Gr1 positive). These results indicated that Mincle positive cellssould play an important role in granuloma formation induced by TDM.

Research Products

• [Journal Article] Sensing necrotic cells (2012)
  • Author(s): Miyake Y, Yamasaki S
  • Journal Title: Adv Exp Med Biol Volume: 738 Pages: 144-52
• [Journal Article] CD169-positive macrophages dominate antitumor immunity by crosspresenting dead cell-associated antigens (2011)
  • Author(s): Asano K, Nabeyama A, Miyake Y, Qiu CH, Kurita A, Tomura M, Kanagawa O, Fujii S, Tanaka M
  • Journal Title: Immunity Volume: 34 Pages: 85-95
  • Peer Reviewed
• [Journal Article] Self and nonself recognition through C-type lectin receptor, Mincle (2010)
  • Author(s): Miyake Y, Ishikawa E, Ishikawa T, Yamasaki S
  • Journal Title: Self Nonself Volume: 1 Pages: 310-313
  • Peer Reviewed
• [Journal Article] Germ-line elimination of electric charge on pre-T-cell receptor(TCR) impairs autonomous signaling for beta-selection and TCR repertoire formation (2010)
  • Author(s): Ishikawa E, Miyake Y, Hara H, Saito T, Yamasaki S
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 19979-84
  • Peer Reviewed
• [Journal Article] xCT deficiency accelerates chemically induced tumorigenesis. (2010)
  • Author(s): Nabeyama A, Kurita A, Asano K, Miyake Y, Yasuda T, Miura I, Nishitai G, Arakawa S, Shimizu S, Wakana S, Yoshida H, Tanaka M
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Pages: 6346-41
  • Peer Reviewed
• [Presentation] Mincle発現細胞の可視化と時期特異的欠損誘導 (2011)
  • Author(s): 三宅靖延、角田茂、岩倉洋一郎、山崎晶
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20110000
• [Presentation] 新規結核菌認識受容体を介する獲得免疫活性化機構の解析 (2011)
  • Author(s): 三宅靖延
  • Organizer: Kyoto T cell Conference
  • Place of Presentation: 京都
  • Year and Date: 20110000
• [Presentation] Mincle is a receptor for mycobacterial glycolipid, cord factor (2011)
  • Author(s): Miyake, Y., and Yamasaki, S
  • Organizer: International Union of Microbiological Societies
  • Place of Presentation: Congress, Sapporo, Japan
  • Year and Date: 20110000
• [Presentation] 死細胞認識受容体Mincleの発現制御の破綻に伴う致死性疾患 (2010)
  • Author(s): 三宅靖延
  • Organizer: BMB
  • Place of Presentation: 神戸
  • Year and Date: 20100000
• [Book] Self/Nonself, Landes Bioscience (2012)
  • Author(s): Miyake, Y
  • Total Pages: 342