The development and clinical application of the novel autoantibody for gastric cancer using proteomic analysis

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790522
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Okayama University (2011); Chiba University (2010)
• Principal Investigator: UMEMURA Hiroshi 岡山大学, 岡山大学病院, 医員 (90456070)
• Project Period (FY): 2010 – 2011
• Keywords: プロテオーム / 癌 / 臨床検査 / 自己抗体 / 腫瘍マーカー

Research Abstract

We constructed a novel proteomic analysis system for the detecting of autoantibodies from sera. This system is composed of first screening procedure of comparative western blotting and subsequent mass analysis. At first, we extracted proteins from four poorly-differenciated gastric cancer cell lines. Using comprehensive western blotting in which protein extracts were used as antigen, we analysed sera from gastric cancer patients and healthy controls. In this analysis, we detected several bands, and identified thirty proteins from these bands with mass spectrometry. Among them, two proteins were successfully validated. These proteins were considered to be novel biomarkers for poorly-differnciated gastric cancer

Research Products

• [Journal Article] Serum level of fibrinogen Aαchain fragment increases in chronic thromboembolic pulmonary hypertension (2011)
  • Author(s): Yano T, Sogawa K, Umemura H, Sakao S, Kasahara Y, Tanabe N, Kodera Y, Takiguchi Y, Tatsumi K, Nomura F
  • Journal Title: Circ J Volume: 75(11) Pages: 2675-82
• [Journal Article] Identification of vitronectin as a novel serum marker for early breast cancer detection using a new proteomic approach (2011)
  • Author(s): Kadowaki M, Sangai T, Nagashima T, Sakakibara M, Yoshitomi H, Takano S, Sogawa K, Umemura H, Fushimi K, Nakatani Y, Nomura F, Miyazaki M
  • Journal Title: J Cancer Res Clin Oncol Volume: 137(7) Pages: 1105-15
• [Journal Article] The measurement of a fibrinogenαC-chain 5. 9kDa fragment(FIC 5. 9) using MALDI-TOF MS and a stable isotope-labeled peptide standard dilution (2011)
  • Author(s): Sogawa K, Kodera Y, Noda K, Ishizuka Y, Yamada M, Umemura H, Maruyama K, Tomonaga T, Yokosuka O, Nomura F
  • Journal Title: Clin Chim Acta Volume: 412(11-12) Pages: 1094-9
• [Journal Article] Identification of a high molecular weight kininogen fragment as a marker for early gastric cancer by serum proteome analysis (2011)
  • Author(s): Umemura H, Togawa A, Sogawa K, Satoh M, Mogushi K, Nishimura M, Matsushita K, Tanaka H, Takizawa H, Kodera Y, Nomura F
  • Journal Title: J Gastroenterol Volume: 46(5) Pages: 577-85
• [Journal Article] Decreases in the serum VLDL-TG/non-VLDL-TG ratio from early stages of chronic hepatitis C : alterations in TG-rich lipoprotein levels (2011)
  • Author(s): Nishimura M, Yamamoto H, Yoshida T, Seimiya M, Sawabe Y, Matsushita K, Umemura H, Sogawa K, Takizawa H, Yokosuka O, Nomura F
  • Journal Title: PLoS One Volume: 6(2) Pages: e17309
• [Journal Article] Increased serum levels of pigment epithelium-derived factor by excessive alcohol consumption-detection and identification by a three-step serum proteome analysis (2011)
  • Author(s): Sogawa K, Kodera Y, Satoh M, Kawashima Y, Umemura H, Maruyama K, Takizawa H, Yokosuka O, Nomura F
  • Journal Title: Alcohol Clin Exp Res Volume: 35(2) Pages: 211-7
• [Journal Article] Effects of humidity on the dried-droplet sample preparation for MALDI-TOF MS peptide profiling (2010)
  • Author(s): Umemura H, Kodera Y, Nomura F
  • Journal Title: Clin Chim Acta Volume: 411(23-24) Pages: 2109-11
• [Journal Article] Reducing the incidence of pseudohyperkalemia by avoiding making a fist during phlebotomy : a quality improvement report (2010)
  • Author(s): Seimiya M, Yoshida T, Sawabe Y, Sogawa K, Umemura H, Matsushita K, Nomura F
  • Journal Title: Am J Kidney Dis Volume: 56(4) Pages: 686-92