2011 Fiscal Year Final Research Report
Development of a novel inspection method to observe intracellular molecular movement : an application of fluorescence correlation spectroscopy(FCS)
| Project/Area Number |
22790531
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
TANAKA Shotaro 東京女子医科大学, 医学部, 助教 (90380667)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 蛍光相関分光法(FCS) / マイクロインジェクション / モノクローナル抗体 |
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| Research Abstract |
Anti-GFP mouse monoclonal antibody was labeled specifically on Fc region by red fluorescent dye. The antibody was injected into HeLa cells that stably expressed PKCβ-GFP. The formation of specific interaction between the antibody and PKCβ-GFP in the cells was demonstrated by analyzing with fluorescence cross-correlation spectroscopy(FCCS), but the interaction completely inhibited the translocation function of PKCβto plasma membrane. To avoid the formation of antibody cross-linking, a Fab fragment was prepared from anti-protein 4.1R antibody expressed by hybridoma, as a new candidate of the present study. Now the antibody function of the Fab fragment is being verified in the cells.
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