2011 Fiscal Year Final Research Report
Individual identification by discrimination of mismatch nucleotides using RNase A
| Project/Area Number |
22790592
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
ASARI Masaru 旭川医科大学, 医学部, 助教 (40360979)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 個人識別 / 一塩基多型 / RNA分解酵素A |
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| Research Abstract |
In 261G/deletion and 796C/A on the human ABO gene, we could genotype the SNP alleles based on the mismatches between template-probe duplexes using synthesized RNA, DNA/RNA chimeric probes, and RNase A. The lengths of probes and the positions of RNA bases corresponding to the SNP affected the sensitivity and specificity of genotyping. Furthermore, the genotypes in two loci were identified by probes designed with the different sizes.
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