2011 Fiscal Year Final Research Report
Application of control method to inhibit pancreatic cancer cell growth and metastasis by the regulation of lumican glycosylation.
| Project/Area Number |
22790675
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | ルミカン / 膵臓癌 / 細胞増殖 / 細胞浸潤 / MMP-9 |
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| Research Abstract |
Lumican expression in the stromal tissues of pancreatic ductal adenocarcinoma(PDAC) correlates with tumor invasion, and tends to correlate with poor prognosis. We used gene transfection techniques to examine the biological roles of lumican secreted from PDAC cells. Lumican-transfected PDAC cells secreted a 70-kDa lumican protein and had an active ERK pathway. Transfection stimulated PDAC cell growth, increased cell adhesion to laminin, inhibited cell invasion, and decreased active matrix metalloproteinase-9. Down-regulation of lumican using siRNA resulted in opposite cell behavior. Thus, the 70-kDa lumican secreted by PDAC cells plays important roles in cell growth and invasion.
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[Journal Article] Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cell-implanted mouse models2011
Author(s)
Matsuda Y, Fujii T, Suzuki T, Yamahatsu K, Kawahara K, Teduka K, Kawamoto Y, Yamamoto T, Ishiwata T, Naito Z.
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Journal Title
J Histochem Cytochem
Volume: 59巻
Pages: 68-75
DOI
Peer Reviewed
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