2011 Fiscal Year Final Research Report
Functional analysis of Ubiquitin ligase Itch in doxorubicin-induced cardiomyopathy
| Project/Area Number |
22790684
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | ドキソルビシン心筋症 / ユビキチン転移酵素Itch |
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| Research Abstract |
In the present study, we focused on the interaction of ubiquitin E3 ligase Itch (a member of Ubiquitin-Proteasome system) and TXNIP to elucidate the mechanism for doxorubicin-induced cardiotoxicity. Knockdown of Itch attenuated doxorubicin-induced TXNIP degradation and subsequent increase in cleaved caspase-3. Furthermore, overexpression of Itch augmented doxorubicin-induced TXNIP degradation and resulted in reduction of cleaved caspase-3. These results suggest that Itch is one of potential target protein to prevent doxorubicin-induced cardiotoxicity.
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