2011 Fiscal Year Final Research Report
Gene therapy for myocardial infarction by using lentivirus encoding kit ligand
| Project/Area Number |
22790717
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kagoshima University |
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Principal Investigator |
HIGUCHI Koji 鹿児島大学, 医歯学総合研究科, 特任助教 (90448580)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 分子心臓病態学 |
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| Research Abstract |
The purpose of this study is to evaluate the effect of kit ligand(KL) isoform against myocardial infarction(MI). The KL expression in transduced cells was confirmed by western blotting and flow cytometry. Endogenous KL-1, KL-2 and c-kit expression in infarcted mouse hearts were evaluated. We found that KL-2 expression in mouse hearts was diminished after MI. Therefor gene therapy using lentivirus encoding KL-2 may improve the prognosis after MI. We were supposed to finish in vivo experiments in this year, however we have difficulties in making large scale virus. We will continue to do in vivo experiments.
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