2011 Fiscal Year Final Research Report
Exploration of micro RNA regulating lung fibrosis and epithelial mesenchymal transition
| Project/Area Number |
22790763
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | 肺線維症 / マイクロRNA |
|---|
| Research Abstract |
To determine the role of epithelial Pten in lung fibrosis, we compared the responses to bleomycin administration between wild-type and bronchioalveolar epithelium-specifically Pten-deleted[SP-C-rtTA/(tetO)_7-Cre/Pten^Δ/Δ(SOPten^Δ/Δ) mouse. Epithelial-derived myofibroblasts were increased in epithelium-specific Pten-deficient mice. Lungs of bleomycin-treated SOPten^Δ/Δmice showed increased pAkt, pS6K, Snai expressions. Akt inactivation definitively saved SOPtenΔ/Δmice. Finally, we detected a reduction of PTEN expression and AKT hyperactivation in the AECs of human IPF lungs. Our results highlight epithelial Pten as a crucial gatekeeper controlling lung fibrosis by modulating integrity of AECs.
|
