2011 Fiscal Year Final Research Report
Directed differentiation of human iPS cells into nephrogenic mesoderm towards regenerative medicine strategy for kidney diseases
| Project/Area Number |
22790786
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
OSAFUNE Kenji 京都大学, iPS細胞研究所, 准教授 (80502947)
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| Project Period (FY) |
2010 – 2011
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| Keywords | iPS細胞 / 中間中胚葉 / 腎臓 / 分化誘導 / 再生 |
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| Research Abstract |
We have succeeded in generating reporter human iPS cell lines for OSR1 gene, which is a specific marker for an embryonic germ layer, the intermediate mesoderm(IM) that gives rise to kidney. By using combinatorial treatment of growth factors, we have established highly efficient differentiation methods to induce human iPS cells into IM cells at an induction rate of more than 90%. We have also confirmed that these human IM cells can differentiate into cells constituting IM derivative organs, such as kidney, adrenal cortex and genitals.
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[Presentation] Induction and monitoring of intermediate mesoderm from human iPSCs and ESCs2011
Author(s)
Mae SI, Shono A, Shiota F, Yasuno T, Nishimura N, Arai S, Takahashi K, Matsubara A, Nakayama N, Ogawa S, McMahon AP, Yamanaka S, Osafune K.
Organizer
9th Annual Meeting, International Society for Stem Cell Research
Place of Presentation
Metro Toronto Convention Centre, Toronto, Ontario, Canada
Year and Date
20110615-18
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