2011 Fiscal Year Final Research Report
Development of EPO-producing cell using immortalize system
| Project/Area Number |
22790792
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 移植・再生医療 / 遺伝子 / エリスロポエチン / 腎性貧血 / 不死化細胞 |
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| Research Abstract |
We developed immortalized cells derived from several cell lines to establish immortalize erythropoietin-producing cells. Erythropoietin production was easily detected using the reporter line system. Erythropoietin-producing cells which expressed GFP were selected by cell sorter. In addition, we developed an erythropoietin-overexpress vector, and confirmed the long-term production of erythropoietin in vector-tranfected cells.
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