2011 Fiscal Year Final Research Report
Identification of the functional role of laminin alpha1 in ischemia.
| Project/Area Number |
22790830
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | Fukushima Medical University |
|---|
Principal Investigator |
TOMIKAWA Naoki 福島県立医科大学, 医学部, 助教 (80468587)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | 神経分子病態学 / 脳梗塞 / 脳髄膜 |
|---|
| Research Abstract |
We hypothesis that the expression of laminin alpha1 is important for mouse brain ischemia and the regeneration. To prove the hypothesis, we made mouse ischemia model and immunostained the brain. Laminin alpha1 did not expression at 6 hour after ischemia and the mainly expression was started at 24 hour after ischemia in the basement membrane of neovessel. Moreover, the expression of laminin alpha1 was observed at 7 days after ischemia which was regeneration stage. Other hand, in the lesion of ischemia, a part of microgila expressed laminin alpha1. These results indicate that laminin alpha1 associates with newly form of brain vessels after brain injury. Next, to examine whether the expression of laminin alpha1 is important for brain injury after ischemia, we generated laminin alpha1 conditional knockout(Lama1 CKO) mouse. We observed the phenotype in Lama1 CKO mice. Lama1 CKO showed markedly behavioral abnormality and we clarified that the abnormality was caused by defect of cerebellum formation using immunohistochemistry, western blot, and slice culture method. On the other hand, the apparent abnormality was not detected in the cerebrum of Lama1 CKO mice compared with wild type mice. These results indicate that it is possible to carry out the operation of ischemia model to Lama1 CKO mice. Finally, as our result, laminin alpha1 associates with the angiogenesis and the accumulation of microglia in encephalopathy and regeneration after ischemia and it is suggested that laminin alpha1 is important for the brain tissue remodeling after ischemia.
|
Research Products
(11 results)
-
[Journal Article] Laminin alpha1 is essential for mouse cerebellar development2012
Author(s)
Ichikawa-Tomikawa N, Ogawa J, Douet V, Xu Z, Kamikubo Y, Sakurai T, Kohsaka S, Chiba H, Hattori N, Yamada Y, Arikawa-Hirasawa E.
-
Journal Title
Matrix Biology
Volume: 31
Pages: 17-28
-
-
-
-
[Journal Article] Perlecan deficiency causes muscle hypertrophy, a decrease in myostatin expression, and changes in muscle fiber composition2010
Author(s)
Xu Z, Ichikawa N, Kosaki Y, Yamada Y, Sasaki T, Sakai LY, Kurosawa H, Hattori N, Arikawa-Hirasawa E.
-
Journal Title
-
[Journal Article] Pigment epithelium-derived factor up-regulation induced by memantine, an N-methyl-d-aspartate receptor antagonist, is involved in increased proliferation of hippocampal progenitor cells2010
Author(s)
Namba T, Yabe T, Gonda Y, Ichikawa N, Sanagi T, Arikawa-Hirasawa E, Mochizuki H, Kohsaka S, Uchino S.
-
Journal Title
Neuroscience
Volume: 167
Pages: 372-383
-
-
-
-
-
