Evaluation of functional roles of estrogen related receptors(ERRs) on differentiation of mesenchymal-derived cells using induced pluripotent stem cells.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22790888
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Saitama Medical University
• Principal Investigator: IJICHI Nobuhiro 埼玉医科大学, 医学部, 研究員 (80380624)
• Project Period (FY): 2010 – 2011
• Keywords: 再生医学 / 発生・分化 / 遺伝子

Research Abstract

To elucidate the functional roles of estrogen related receptors(ERRs) on the reprogramming and differentiation of mesenchymal-derived cells, we examined combined methods achieving efficient reprogramming and mesenchymal-derived cell differentiation using viral vector systems. This made a partly improved efficiency of differentiation, while it was demonstrated that differentiation potential of iPS cells varied widely between clones. In this study, we established the basis for evaluation of ERR functions in reprogramming and differentiation, while these results suggest that generation of genetically and functionally homogenous iPS cells is necessary for in vivo evaluation of gene function as well as clinical application of iPS cells.

Research Products

• [Journal Article] Association of double-positive FOXA1 and FOXP1 immunoreactivities with favorable prognosis of tamoxifen-treated breast cancer patients (2012)
  • Author(s): Ijichi N, Shigekawa T, Ikeda K, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S
  • Journal Title: Horm Cancer
  • DOI: DOI:10.1007/s12672-012-0111-0
  • Peer Reviewed
• [Journal Article] Forkhead box transcription factor, forkhead box A1, shows negative association with lymph node status in endometrial cancer, and represses cell proliferation and migration of endometrial cancer cells (2012)
  • Author(s): Abe Y, Ijichi N, Ikeda K, Kayano H, Horie-Inoue K, Takeda S, Inoue S
  • Journal Title: Cancer Sci Volume: 103 Pages: 806-812
  • Peer Reviewed
• [Journal Article] FOXP1, an estrogen-inducible transcription factor, modulates cell proliferation in breast cancer cells and 5-year recurrence-free survival of patients with tamoxifen-treated breast cancer (2011)
  • Author(s): Shigekawa T, Ijichi N, Ikeda K, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S
  • Journal Title: Horm Cancer Volume: 2 Pages: 286-297
  • Peer Reviewed
• [Presentation] ER陽性乳癌におけるフォークヘッド転写因子FOXP1の役割 (2012)
  • Author(s): 重川崇、伊地知暢広、池田和博、堀江公仁子、清水千佳子、佐治重衡、青儀健二郎、津田均、大崎昭彦、佐伯俊昭、井上聡
  • Organizer: 第12回関東ホルモンと癌研究会
  • Place of Presentation: 文京区
  • Year and Date: 2012-01-21
• [Presentation] 乳がんならびに子宮内膜がんにおけるフォークヘッド転写因子FOXA1の機能と臨床的意義 (2011)
  • Author(s): 伊地知暢広、重川崇、阿部弥生、池田和博、堀江公仁子、茅野秀一、津田均、大崎昭彦、竹田省、佐伯俊昭、井上聡
  • Organizer: 第19回日本ステロイドホルモン学会
  • Place of Presentation: 福岡市
  • Year and Date: 2011-11-26
• [Book] Roles of Estrogen-Related Receptors in Adipocyte Differentiation. Adipocytes : Biology, Regulation and Health Impact (2012)
  • Author(s): Ijichi N, Ikeda K, Horie-Inoue K, Inoue S
  • Publisher: New York : Nova Science Publishers(At press)