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2012 Fiscal Year Final Research Report

Investigation of the molecular mechanisms of constitutive NF-kBactivation for the identification of therapeutic targets of adult T-cell leukemia cells

Research Project

  • PDF
Project/Area Number 22790905
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

SAITOH Yasunori  東京医科歯科大学, 医歯学総合研究科・ウイルス制御学, 助教 (00516312)

Project Period (FY) 2010 – 2012
Keywords血液腫瘍学
Research Abstract

Adult T-cell leukemia (ATL) is a T-cell neoplasm caused by Human T-cell leukemia virus type I infection. Previous reports described that the ubiquitin-editing enzyme A20 protects cytokine- or NK-induced cell death of endothelial cells via inhibiting caspase-8 activation. I found that the A20 protein and mRNA expressions were detectable in ATL cells in contrast to some of B-cell lymphoma cell lines with a somatic mutation and/or deletion of A20. Interestingly, quantitative RT-PCR analysis revealed that the A20 mRNA expression was frequently up-regulated in PBMCs derived from ATL patients. And A20 was found to be required for the growth of ATL-derived and HTLV-I-transformed cells. RNA-interference mediated knock-down of A20 induced the activation of apoptosis initiator caspase-8 concomitant with the activation of downstream effector caspase-3/7. In addition, I found that A20 physically interacts with caspase-8. Importantly, depletion of A20 suppressed the growth of ATL cells and increased the annexin V-positive population. These results suggest that A20 contributes to the survival of ATL cells and implicates A20 as a therapeutic molecular target for ATL therapy.

  • Research Products

    (12 results)

All 2012 2011 2010 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (7 results) Book (1 results) Remarks (1 results)

  • [Journal Article] An IκB kinase 2 inhibitor IMD-0354 suppresses the survival of adult T-cell leukemia cells2012

    • Author(s)
      Uota S, Zahidunnabi Dewan M, Saitoh Y, Muto S, Itai A, Utsunomiya A, Watanabe T, Yamamoto N, Yamaoka S
    • Journal Title

      Cancer Sci

      Volume: 103 Pages: 100-106

    • Peer Reviewed
  • [Journal Article] Neutrophil extracellular traps mediate a host defense response to human immunodeficiency virus-12012

    • Author(s)
      Saitoh T, Komano J, Saitoh Y, Misawa T, Takahama M, Kozaki T, Uehata T, Iwasaki H, Omori H, Yamaoka S, Yamamoto N, Akira S
    • Journal Title

      Cell HostMicrobe

      Volume: 12 Pages: 109-116

    • Peer Reviewed
  • [Journal Article] Overexpression of NF-κB inducing kinase underlies constitutive NF-κB activation in lung cancer cells2010

    • Author(s)
      Saitoh Y, Martinez Bruyn VJ, Uota S, Hasegawa A, Yamamoto N, Imoto I, Inazawa J, Yamaoka S
    • Journal Title

      Lung Cancer

      Volume: 70 Pages: 263-270

    • Peer Reviewed
  • [Presentation] HTLV-I感染細胞におけるA20は細胞生存と造腫瘍能に寄与する2012

    • Author(s)
      斉藤愛記、掛谷綾香、深澤麻純、大迫美穂、宇野雅哉、宇都宮與、渡邉俊樹、山岡昇司
    • Organizer
      新学術領域研究「修飾シグナル病」2012年度修飾シグナル病若手ワークショップ
    • Place of Presentation
      湯河原温泉「ホテルあかね」
    • Year and Date
      20121003-1005
  • [Presentation] ユビキチン化修飾酵素A20はHTLV-I感染細胞の生存に寄与する2012

    • Author(s)
      齋藤愛記
    • Organizer
      第1回ATLシンポジウム
    • Place of Presentation
      東京大学医科学研究所講堂
    • Year and Date
      2012-08-25
  • [Presentation] ユビキチン化修飾タンパク質A20はHTLV-1感染細胞株の増殖に重要である2011

    • Author(s)
      掛谷綾香、深澤麻純、魚田慎、藤井雅寛、斉藤愛記、山岡昇司
    • Organizer
      第4回HTLV-1研究会/合同班会議
    • Place of Presentation
      東京大学弥生講堂
    • Year and Date
      20110918-19
  • [Presentation] Deregulated expression of NF- κ B inducing kinase contributes to constitutive NF- κ B activation andgrowth properties of ovarian cancer cells2011

    • Author(s)
      Masaya Uno, Yasunori Saitoh, Shin Uota, Toshiro Kubota, Shoji Yamaoka
    • Organizer
      Cincinnati Cancer Symposium Series:2011 Symposium on NF-KB, Cancer, Obesity, and Inflammation
    • Place of Presentation
      米国オハイオ州シンシナティ
    • Year and Date
      2011-05-24
  • [Presentation] ユビキチン化修飾酵素A20はNF-κB inducing kinase によるNF-κB活性化を増強する2011

    • Author(s)
      齊藤愛記
    • Organizer
      文部科学省・科学研究費補助金・新学術領域研究「修飾シグナル病」第一回公開シンポジウム
    • Place of Presentation
      東京大学医科学研究所1号館1F講堂
    • Year and Date
      2011-01-29
  • [Presentation] ユビキチン化修飾酵素A20はNF-κB inducing kinaseによるNF-κB活性化を増強する2011

    • Author(s)
      斉藤愛記
    • Organizer
      第一回公開シンポジウム「修飾シグナル病」学術領域の創出
    • Place of Presentation
      東京大学医科学研究所講堂
    • Year and Date
      2011-01-29
  • [Presentation] A20はNIKによるNF-κB活性化を増強する2010

    • Author(s)
      斉藤愛記
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会・合同大会
    • Year and Date
      2010-12-08
  • [Book] ウイルス発がんとNF-κBシグナル伝達の制御異常2012

    • Author(s)
      斉藤愛記,山岡昇司
    • Total Pages
      783-788
    • Publisher
      羊土社
  • [Remarks]

    • URL

      http://molv.org/

URL: 

Published: 2014-08-29  

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