Dissecting the Factors Involved in the Locomotion Mode of Neuronal Migration in the Developing Cerebral Cortex

2012 Fiscal Year Final Research Report

• Project/Area Number: 22790998
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Doshisha University (2011-2012); Institute for Developmental Research, Aichi Human Service Center (2010)
• Principal Investigator: NISHIMURA Yoshiaki 同志社大学, 高等研究教育機構, 助教 (50508603)
• Project Period (FY): 2010 – 2012
• Keywords: 発達小児科学

Research Abstract

Neuronal migration is essential for proper cortical layer formation, as migration defects result in neurological disorders. The locomotion mode of neuronal migration covers most of the migration route and thereby is the main contributor to cortical layer formation. However, analysis of the molecular mechanisms regulating this mode is difficult. In this study, we used an ex vivo chemical inhibitor screening, allowing us to directly analyze the locomotion mode of migration, and found several regulatory molecules regurating the locomotion mode.

Research Products

• [Journal Article] Biochemical and morphological characterization of MAGI-1 in neuronal tissue (2012)
  • Author(s): Hidenori Ito, Rika Morishita, Kaori Sudo, Yoshiaki V. Nishimura, Yutaka Inaguma, Ikuko Iwamoto, Koh-ichi Nagata
  • Journal Title: J Neurosci Res. Volume: 90(9) Pages: 1776-81
  • DOI: doi: 10.1002/jnr.23074.
  • Peer Reviewed
• [Journal Article] Application of in utero electroporation and live imaging in the analyses of neuronal migration during mouse brain development (2012)
  • Author(s): Yoshiaki V. Nishimura, Tomoyasu Shinoda, Yutaka Inaguma, Hidenori Ito and Koh-ichi Nagata
  • Journal Title: Med Mol Morphol Volume: 45(1) Pages: 1-6
  • DOI: doi: 10.1007/s00795-011-0557-0.
  • Peer Reviewed
• [Journal Article] Dissecting the Factors Involved in the Locomotion Mode of Neuronal Migration in the Developing Cerebral Cortex (2010)
  • Author(s): Yoshiaki V. Nishimura, Katsutoshi Sekine, Kaori Chihama, Kazunori Nakajima, Mikio Hoshino, Yo-ichi Nabeshima, and Takeshi Kawauchi
  • Journal Title: J Biol Chem. Volume: 285(8) Pages: 5878-87
  • DOI: doi: 10.1074/jbc.M109.033761.
  • Peer Reviewed
• [Presentation] Neurovascular interaction in the developing neocortex (2012)
  • Author(s): Yoshiaki V. Nishimura, Yutaka Inaguma, Masatsugu Ema, Mitsuharu Hattori, Koh-ichi Nagata, Ken-ichi Mizutani
  • Organizer: 第20回日本血管生物医学会学術集会(The 10th Korea-Japan Joint Symposium on Vascular Biology)
  • Place of Presentation: 徳島
  • Year and Date: 20121205-07
• [Presentation] Essential role of SIL1, a causative gene of Marinesco-Sjogren syndrome, in the neuronal migration during corticogenesis (2012)
  • Author(s): Koh-ichi Nagata, Yutaka Inaguma, Yoshiaki V. Nishimura, Ayako Taguchi, Hidenori Ito, Motomasa Suzuki, Masanori Hosokawa, Toshiyuki Kumagai
  • Organizer: Society for Neuroscience 42nd Annual Meeting
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 20121013-17
• [Presentation] Molecular and cellular mechanisms for the locomotion mode and terminal translocation mode of neuronal migration (2012)
  • Author(s): Takeshi Kawauchi, Yoshiaki V. Nishimura, Mima Shikanai, Koh-ichi Nagata, Kazunori Nakajima
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] Cdk5 plays multiple roles in cortical neuronal migration and morphological changes (2012)
  • Author(s): Takeshi Kawauchi, Yoshiaki V. Nishimura, Mima Shikanai, Koh-ichi Nagata, and Kazunori Nakajima
  • Organizer: 第45回日本発生生物学会・第64 回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20120528-31
• [Presentation] Angiogenesis in the developing cerebral cortex (2011)
  • Author(s): Yoshiaki V. Nishimura, Yutaka Inaguma, Ayako Taguchi, Hidenori Ito and Koh-ichi Nagata
  • Organizer: 第19回日本血管生物医学会学術集会(The 1st Asia-Pacific Vascular Biology Meeting)
  • Place of Presentation: 東京
  • Year and Date: 20111208-10
• [Presentation] Functional analysis of Dysbindin, a schizophrenia risk factor, in dendritic spine formation (2011)
  • Author(s): Hidenori Ito, Rika Morishita, Yoshiaki Nishimura, Tomoyasu Shinoda, Ikuko Iwamoto, Koh-ichi Nagata.
  • Organizer: 2011 American Society for Cell Biology Annual Meeting
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 20111203-07
• [Presentation] 神経突起伸長におけるMAGI-1 の機能解析 (2011)
  • Author(s): 伊東秀記、森下理香、西村嘉晃、篠田友靖、 岩本郁子、須藤香織、永田浩一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 20110921-24
• [Presentation] ライブイメージングを用いた発生期大脳皮質における神経細胞移動の制御機構の解析 (2011)
  • Author(s): 西村嘉晃、星野幹雄、永田浩一、仲嶋一範、川内健史
  • Organizer: 第43回日本臨床分子形態学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20110909-10
• [Presentation] Role of the Septin in neural cell migration during brain development (2011)
  • Author(s): Koh-ichi Nagata, Tomoyasu Shinoda, Yoshiaki V. Nishimura, Hidenori Ito
  • Organizer: International Society for Neurochemistry-European Society for Neurochemistry 23rd Biennial Meeting
  • Place of Presentation: Athens, Greece
  • Year and Date: 20110828-0901
• [Presentation] Possible involvement of Rap1 signaling and septins in angiogenesis and brain development (2011)
  • Author(s): 西村嘉晃、稲熊裕、田口紋子、伊東秀記、永田浩一
  • Organizer: 第2回「血管と神経」新学術領域会議
  • Place of Presentation: 熊本
  • Year and Date: 20110818-20
• [Presentation] Regulation of radial migration of cortical pyramidal neurons by a Ca2+/calmodulin-dependent protein kinase I cascade (2011)
  • Author(s): Shin-ichiro Horigame, Sayaka Takemoto-Kimura, Aki Adachi-Morishima, Natsumi Ageta-Ishihara, Kanzo Suzuki, Mio Nonaka, Michiko Okamura, Yoshiaki V. Nishimura, Takeshi Kawauchi, Kazunori Nakajima, Hiroyuki Okuno, Haruhiko Bito
  • Organizer: 第84回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20110322-24
• [Presentation] Role of Septins in neural cell migration during brain development-Screening of novel interacting partners (2011)
  • Author(s): Tomoyasu Shinoda, Yoshiaki Nishimura, Hidenori Ito, Koh-ichi Nagata
  • Organizer: EMBO Workshop "Function and structure of septins, filament-forming GTP-binding proteins"
  • Place of Presentation: St. Goar, Germany
  • Year and Date: 20110306-09
• [Presentation] Analyzing the mechanisms of the morphological changes of locomoting neurons in the developing cerebral cortex (2010)
  • Author(s): Yoshiaki V. Nishimura, Koh-ichi Nagata, Yo-ichi Nabeshima, Mikio Hoshino, Kazunori Nakajima, Takeshi Kawauchi
  • Organizer: 第33回日本分子生物学会年会・第83 回日本 生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] Dissecting the factors involved in the morphological changes of locomoting neurons in the developing cerebral cortex (2010)
  • Author(s): Yoshiaki Nishimura, Katsutoshi Sekine, Kaori Chihama, Koh-ichi Nagata, Kazunori Nakajima, Mikio Hoshino, Yo-ichi Nabeshima, Takeshi Kawauchi
  • Organizer: 第33回日本神経科学大会、第53 回日本神経 化学会大会、第20 回日本神経回路学会大会合 同大会(Neuro2010)
  • Place of Presentation: 神戸
  • Year and Date: 20100902-04
• [Presentation] 大脳皮質形成過程におけるロコモーション移動を制御する分子機構の解析 (2010)
  • Author(s): 西村嘉晃、関根克敏、地濱香央里、永田浩一、仲嶋一範、星野幹雄、鍋島陽一、川内健史
  • Organizer: 第74回日本生化学会中部支部例会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-05-29