Identification of tumor stem-specific transcription factor in neuroblastoma sphere

2012 Fiscal Year Final Research Report

• Project/Area Number: 22791017
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: TAKENOBU Hisanori 千葉県がんセンター(研究所), 発がん制御研究部, 研究員 (60392247)
• Project Period (FY): 2010 – 2012
• Keywords: 幹細胞 / 発がん / 分化

Research Abstract

We reported that CD133 suppressed differentiation in NB cells and CD133 was up-regulated in NB sphere (ONCOGENE, 2011). To identify the sphere specific transcription factors, we examined expression of several transcription factors in NB spheres. The expression pattern of the TFX transcription factor was correlated with that of CD133 during NB tumor sphere formation. Furthermore, TFX directly bound to CD133 promoter region and up-regulated CD133 expression, cell proliferation and NB sphere forming efficiency. These results suggest that tumor sphere specific transcription factor TFX may contribute to maintenance of tumor initiating cells and their characteristics.

Research Products

• [Journal Article] CD133 suppresses neuroblastoma cell differentiation via signal pathway modification (2011)
  • Author(s): Takenobu H, Shimozato 0, Nakamura T, Ochiai H, Yamaguchi Y, Ohira M, Nakagawara A, Kamijo T
  • Journal Title: Oncogene 2011 Volume: 6;30(1) Pages: 97-105
• [Journal Article] Polycomb group molecule PHC3 regulates polycomb complex composition and prognosis of osteosarcoma
  • Author(s): Iwata S, Takenobu H, Kageyama H, Koseki H, Ishii T, Nakazawa A, Tatezaki S, Nakagawara A, Kamijo T
  • Journal Title: Cancer Sci Volume: 101(7) Pages: 1646-1652
• [Journal Article] HDM2 impairs Noxa transcription and affects apoptotic cell death in a p53/p73-dependentmanner in neuroblastoma
  • Author(s): Shi Y, Takenobu H, Kurata K, Yamaguchi Y, Yanagisawa R, Ohira M, Koike K, Nakagawara A, Jiang LL, Kamijo T
  • Journal Title: Eur J Cancer Volume: 46(12) Pages: 2324-2334
• [Journal Article] Bmil regulates cell fate via tumor suppressor WWOX repression in small-cell lung cancer cells
  • Author(s): Kimura M, Takenobu H, Akita N, Nakazawa A, Ochiai H, Shimozato 0, Fujimura Y, Koseki H, Yoshino I, Kimura H, Nakagawara A, Kamijo T
  • Journal Title: Cancer Sci Volume: 102(5) Pages: 983-990
• [Presentation] Identification of key molecules that regulate neuroblastoma sphere formation (2012)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,上原悠,藁谷美雪,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20121100
• [Presentation] CD133 suppresses neuro-blastoma cell differentiation via signal pathway modification, (2012)
  • Author(s): 竹信尚典
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20121100
• [Presentation] Tumor sphere specific CD 133regulation in neuroblastoma (2012)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,上原悠,藁谷美雪,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120900
• [Presentation] Tumor sphere specific CD133 regulation in neuroblastoma (2012)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,上原悠,藁谷美雪,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Organizer: Advances of Neuroblastoma Research 2012
  • Place of Presentation: トロント
  • Year and Date: 20120600
• [Presentation] 神経芽腫/脳腫瘍スフェア特異的なCD133発現調節機構 (2011)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Organizer: 第53回日本小児血液・がん学会
  • Place of Presentation: 前橋
  • Year and Date: 20111100
• [Presentation] CD133 plays roles in keeping the undifferentiated status of human colon cancer cells via PI3K/AKT activation (2011)
  • Author(s): 竹信尚典,下里修,秋田直洋,山口陽子,吉田早哉香,中川原章,上條岳彦
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111000
• [Presentation] 幹細胞マーカーCD133は細胞内シグナル活性化を介して神経芽腫の増殖と分化を制御する (2011)
  • Author(s): 竹信尚典,上條岳彦
  • Organizer: 第116回小児血液腫瘍懇話会
  • Place of Presentation: 東京
  • Year and Date: 20110500
• [Presentation] CD133はシグナル経路依存的に受容体RETの転写抑制を引き起こし、神経芽腫の増殖と分化を制御する (2010)
  • Author(s): 竹信尚典,下里修,山口陽子,秋田直洋,吉田早哉香,落合秀匡,大平美紀,中川原章,上條岳彦
  • Place of Presentation: 大阪
  • Year and Date: 20101200
• [Presentation] CD133 regulates neuroblastoma stemness via p38MAPK and PI3K/Akt activation (2010)
  • Author(s): 竹信尚典,下里修,落合秀匡,山口陽子,大平美紀,中川原章,上條岳彦
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 20100900
• [Presentation] CD133 regulates signal transduction pathways and prevents differentiation via RET suppression in neuroblastoma cells (2010)
  • Author(s): 竹信尚典,下里修,落合秀匡,山口陽子,大平美紀,中川原章,上條岳彦
  • Organizer: Advances of Neuroblastoma Research 2010
  • Place of Presentation: ストックホルム
  • Year and Date: 20100600
• [Remarks]
  • URL: http://www.chiba-cc.jp/inst/jp/organization/005.htmlhttp://kamijo-labo.jp/