2011 Fiscal Year Final Research Report

Analysis of constriction mechanisms in response to oxygen in the ductus venosus and umbilical vessels-The role of sarcoplasmic reticulum and Rho kinases-

Research Project

Project/Area Number	22791040
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Embryonic/Neonatal medicine
Research Institution	Tokyo Women's Medical University
Principal Investigator	TAKEUCHI Daiji 東京女子医科大学, 医学部, 助教 (40328456)
Project Period (FY)	2010 – 2011
Keywords	臍帯動脈 / 静脈管 / 酸素 / Rhoキナーゼ / Ca
Research Abstract	Gene expression related to vascular constriction/dilation in rabbit umbilical arteries and veins was analyzed by real-time quantitative PCR. Expression levels of RhoB and Rho kinase 1 in the umbilical vessels were higher than those of RhoA and Rho kinase 2, respectively. Expression levels of Rho pathway genes in umbilical vessels during fetal development were relatively stable. Gene expression related to the regulation/storage of Ca^<2+> in the sarcoplasmic reticulum(SR) and the contraction proteins was increased as fetal development. Expression levels of the SR Ca^<2+> regulation/storage protein genes varied among these umbilical vessels. Since the content and constitution of SR proteins involved in Ca^<2+> regulation varied during fetal development, the SR activity of the Ca^<2+> release/storage might be differ according to the umbilical vessel and the developmental stage.

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