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2012 Fiscal Year Final Research Report

Functional analysis of Periostin in malignant melanoma

Research Project

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Project/Area Number 22791100
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research Institution独立行政法人医薬基盤研究所

Principal Investigator

SERADA Satoshi  独立行政法人医薬基盤研究所, 創薬基盤研究部, 研究員 (50463302)

Project Period (FY) 2010 – 2012
Keywords癌 / periostin
Research Abstract

Malignant melanoma is one of the most aggressive cancers, since this tumor metastasize to various organs and resistant to chemotherapy. By using quantitative proteomic apProach, we have been identified that Peroostin, one of the extracellular matrix proteins, was highly expressed in the melanoma tissue compared with normal skin tissue. This study was aimed to reveal the association between Periostin and malignant melanoma.
Recombinant Periostin protein treatment significantly accelerated the proliferation of nlelanoma cell lines (Mewo, G361 and VMRC-MELG). This growth promoting effect induced by Periostin treatment was dependent on the integrin/P44/42 MAPK signaling Pathway in vitro. To reveal the role of Periostin to the growth of the malignant nlelanoma, Periostin/RAG2 double knockout mice were developed by mating Periostin deficient mice and RAG2 deficient mice. In Periostin knockout mice and Periostin/RAG2 double knockout mice, Mewo cells were subcutaneously implanted and tumor proliferation was analyzed. Compared with Periostin knockout mice, the tumor proliferation of Mewo was significantly delayed in Periostin/RAG2 double knockout mice, demonstrating that Periostin also induce proliferation of melanoma cells in vivo. These results suggest that Periostin induces proliferation of tumor cells in vi tzO and in vivo and Periostin would be a promising therapeutic target of malignant melanoma.

  • Research Products

    (21 results)

All 2012 2011 2010

All Journal Article (11 results) Presentation (8 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Periostin Facilitates Skin Sclerosis via PI3K/Akt Dependent Mechanism in a Mouse Model of Scleroderma2012

    • Author(s)
      Yang L, Serada S, Fujimoto M, Terao M, Kotobuki Y, Kitaba S, Matsui S, Kudo A, Naka T, Murota H, Katayama I
    • Journal Title

      PLoS One

      Volume: 7(7) Pages: e41994

  • [Journal Article] Periostin, a matricellular protein2012

    • Author(s)
      Ontsuka K, Kotobuki Y, Shiraishi H, Serada S, Ohta S, Tanemura A, Yang L, Fujimoto M, Arima K, Suzuki S, Murota H, Toda S, Kudo A, Conway S, Narisawa Y, Katayama I, Izuhara K, Naka T
    • Journal Title

      accelerates cutaneous wound repair by activating dermal fibroblasts Experimental Dermatology

      Volume: 21(5) Pages: 331-336

  • [Journal Article] Dysregulation of Melanocyte Function by Th17-related Cytokines: Significance of Th17 Cell Infiltration in Autoimmune Vitiligo Vulgaris2012

    • Author(s)
      Kotobuki Y, Tanemura A, Yang L, Itoi S, Kaneda M, Murota H, Fujimoto M, Serada S, Naka T Katayama I
    • Journal Title

      Pigment Cell Melanoma Res

      Volume: 25(2) Pages: 219-30

  • [Journal Article] Anti-proliferative effect of SOCS-1 through the suppression of STAT3 and p38 MAPK activation in gastric cancer cells2012

    • Author(s)
      Souma Y, Nishida T, Serada S, Iwahori K, Takahashi T, Fujimoto M, Ripley B, Nakajima K, Miyazaki Y, Mori M, Doki Y, Sawa Y, Naka T
    • Journal Title

      Int J Cancer

      Volume: 131(6) Pages: 1287-96

  • [Journal Article] Serum leucine-rich alpha-2 glycoprotein is a disease activity biomarker in ulceratiVe colitis2012

    • Author(s)
      Serada S, Fujimoto M, Terabe F, Iijima H, Shinzaki S, Matsuzaki S, Ohkawara T, Nezu R, Nakajima S, Kobayashi T, Plevy SE, Takehara T, Naka T
    • Journal Title

      Inflamm Bowel Dis

      Volume: 18(11) Pages: 2169-79

  • [Journal Article] Blockade of Interleukin-6 Signaling Suppresses Not Only Th17 But Also Interphotoreceptor Retinoid Binding Protein-Specific Thl By Promoting Regulatory T cell In Experimental Autoimmune Uveoretinitis2011

    • Author(s)
      Haruta H, Ohguro N, Fujimoto M, Hohki S, Terabe F, Serada S, Nomura S, Nishida K, Kishimoto T, Naka T
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 52 Pages: 3264-71

  • [Journal Article] The Influence of Excessive IL-6 Production In Vivo on the Development and Function of Foxp3+ Regulatory T Cells2011

    • Author(s)
      Fujimoto M, Nakano M, Terabe F, Kawahata H, Ohkawara T, Han Y, Ripley B, Serada S, Nishikawa T, Kimura A, Nomura S, Kishimoto T, Naka T
    • Journal Title

      J Immunol

      Volume: 186(1) Pages: 32-40

  • [Journal Article] Overexpression of SOCS3 exhibits preclinical antitunlor activity against malignant pleural mesothelioma2011

    • Author(s)
      Iwahori K, Serada S, Fujimoto M, Nomura S, Osaki T, Chun Man Lee, Mizuguchi H, Takahashi T, Ripley B, Okumura M, Kawase I, Kishimoto T, Naka T
    • Journal Title

      Int J Cancer

      Volume: 129(4) Pages: 1005-17

  • [Journal Article] Comparative analysis of the effects of anti-IL-6 receptor mAb and anti-TNF mAb treatment on CD4+ T-cell responses in murine colitis2011

    • Author(s)
      Terabe F, Fujimoto M, Serada S, Shinzaki S, Iijima H, Tsujii M, Hayashi N, Nomura S, Kawahata H, Myoung Ho Jang, Miyasaka M, Mihara M, Ohsugi Y, Kishimoto T, Naka T
    • Journal Title

      Inflamm Bowel Dis

      Volume: 17(2) Pages: 491-502

  • [Journal Article] Blockade of Interleukin-6 Signaling SupPresses Experimental Autoimmune Uveoretinitis by the Inhibition of Inflammatory Th17 Responses2010

    • Author(s)
      Hohki S, Ohguro N, Haruta H, Nakai K, Terabe F, Serada S, Fujimoto M, Nomura S, Kawahata H, Kishimoto T, Naka T
    • Journal Title

      Exp Eye Res

      Volume: 91(2) Pages: 162-70

  • [Journal Article] iTRAQ-based proteomic identification of leucine rich alpha 2 glycoprotein (LRG) as a novel inflammatory biomarker in autoimmune diseases2010

    • Author(s)
      Serada S, Fujimoto M, Ogata A, Terabe F, Hirano T, Iijima H, Shinzaki S, Nishikawa T, Ohkawara T, Iwahori K, Ohguro N, Kishimoto T, Naka T
    • Journal Title

      Ann Rheum Dis

      Volume: 69 Pages: 770-774

  • [Presentation] 定量的リン酸化プロテオーム解析による消化管間質腫瘍のイマチニブ耐性因子の探索2012

    • Author(s)
      世良田聡,高橋剛,浦瀬真伊子,藤本穣,原田絵美,西田俊朗,仲哲治
    • Organizer
      第10回日本ヒトプロテオーム学会
    • Place of Presentation
      本科学未来館7階(東京)
    • Year and Date
      20120726-27
  • [Presentation] Heterogeneous Nuclear RNP-K Is a Novel Cold-Related Autoantigen in Patients with Raynaud' s Phenomenon2012

    • Author(s)
      Satoshi Serada, Minoru Fujimoto, Tetsuji Naka
    • Organizer
      ACR/ARHP Annual Meeting, in Washington, DC, November 09-14, 2012. November 12 (ACR 2012第78回米国リウマチ学会議)
    • Place of Presentation
      米国(ワシントン)
    • Year and Date
      20120000
  • [Presentation] Quantitative proteomic analysis of tumor growth associated proteins in cutaneous malignant melanoma Short title: Proteomics of melanoma2012

    • Author(s)
      Satoshi Serada, Yorihisa Kotobuki, Atsushi Tanemura, Ichiro Katayama, Tetsuji Naka
    • Organizer
      103rd Annual Meeting of the American Association for Cancer Research;2012
    • Place of Presentation
      米国(シカゴ)
    • Year and Date
      2012-04-02
  • [Presentation] Periostin, a matricellular protein, accelerates wound repair by activating dermal fibroblasts2011

    • Author(s)
      Yorihisa Kotobuki, Kanako Ontsuka, Satoshi Serada, Hiroshi Shiraishi, Atsushi Tanemura, Shoichiro Ohta, Lingli Yang, Tetsuji Naka, Kenji Izuhara, Ichiro Katayama
    • Organizer
      第36回日本研究皮膚科学会学術大会・総会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      20111209-11
  • [Presentation] Leucine rich alpha 2 glycoprotein (LRG) is a novel biomarker for monitoring disease activity in patients with Ulcerative Colitis2011

    • Author(s)
      Satoshi Serada, Minoru Fujimoto, Fumitaka Terabe, Tetsuji Naka
    • Organizer
      第40回日本免疫学会総会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Year and Date
      20111127-29
  • [Presentation] 定量的プロテオーム解析による子宮内膜癌抗原蛋白質の探索2011

    • Author(s)
      世良田聡,横山拓平,榎本隆之,松崎慎哉,森本晶子,金雅子,藤本穣,木村正,仲哲治
    • Organizer
      第9回 日本プロテオーム学会
    • Place of Presentation
      新潟市朱鷺メッセ2階(新潟市)
    • Year and Date
      20110728-29
  • [Presentation] 自己免疫疾患の新規活動性マーカーとしてのleucine rich alpha 2 glycoprotein2010

    • Author(s)
      世良田聡、藤本穣、寺部文隆、西川哲平、仲哲治
    • Organizer
      第8回 日本プロテオーム学会
    • Place of Presentation
      東京ベイホテル東急(浦安市)
    • Year and Date
      20100726-27
  • [Presentation] lLabotatory For Immune Signal, National Institute of Biomedical Innovation, Ibaraki City (Osaka)/JAPAN, 2 Surgery, Osaka University, Suita/JAPAN, 3 Surgery, Osaka Police Hospital, Osaka/JAPAN, 4 Laboratory For Immune Signal, National Institute of Biomedical Innovation, Ibaraki City (Osaka)/JAPAN2010

    • Author(s)
      S. Serada, Y. Souma, T. Nishida, M. Fujimoto, T. Naka
    • Organizer
      SOCS-1,-3 GENE DELIVERY IN GASTRIC CANCER CELLS INDUCES A POTENT ANTI-PROLIFERATIVE EFFECT VIA THE SUPPRESSION OF JAK/STAT AND P38 MAPK S IGNAL ING PATHWAYS
    • Place of Presentation
      イタリア(ミラノ)
    • Year and Date
      2010-10-09
  • [Book] サイトカインシグナル伝達抑制分子(SOCS)の新規抗癌剤としての応用2010

    • Author(s)
      世良田聡,仲哲治
    • Total Pages
      602-607
    • Publisher
      医歯薬出版株式会社, 医学のあゆみ,234(5)
  • [Patent(Industrial Property Rights)] 創傷治癒剤2010

    • Inventor(s)
      寿 順久、世良田 聡、仲 哲治
    • Industrial Property Rights Holder
      財団法人ヒューマンサイエンス振興財団
    • Industrial Property Number
      特許権、特願2010-151139号
    • Filing Date
      2010-07-01

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Published: 2014-08-29  

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