2011 Fiscal Year Final Research Report
Attempt of developing a technique for detecting in situ protein. glutathionylation and its adaptation to the studies of psychiatric disorders.
| Project/Area Number |
22791114
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | 酸化ストレス / redox反応 / 精神疾患 / in vivo検出法 / グルタチオン化 / 薬物依存 |
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| Research Abstract |
It is postulated that oxidative stress may be involved in the pathophysiology of some psychiatric disorders, however, sophisticated method to investigate redox status in brain is lacking even in animal studies. In this study, we aimed to confirm the alteration in redox status induced by repeated cocaine administration in situ in the rat striatum. First, we found that an anti-oxidative stress compound, N-acetylcysteine, reduced many synaptic proteins in the nucleus accumbens as the consequence of repeated cocaine administration. Second, it appeared that the production of glutathione, the major endogenous antioxidant, shifted from astroglias to neurons. These results suggest a necessity of more cell-type-specific approach to detect the alteration of glutathione metabolism.
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