2011 Fiscal Year Final Research Report
GRP78 is a novel target for radiation therapy in malignant glioma
| Project/Area Number |
22791170
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
NODA Shinei 群馬大学, 医学部, 助教 (60396645)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Keywords | 神経膠芽腫 / GRP78 / 放射線治療 |
|---|
| Research Abstract |
Stable knockdown cell lines of GRP78 using lentiviral based sh-RNA transduction were generated in 3 glioblastoma (GBM) cell lines. GBM cell lines were exposed 6 Gy of gamma radiation and subsequently evaluated to determine the extent of double strand DNA damage using comet and gamma-H2Ax assay and the radiosensitivity using colony formation assay and MTS assay. Apoptosis was evaluated using flowcytometry and westernblot assay.
|
Research Products
(3 results)
