2011 Fiscal Year Final Research Report
GRP78 is a novel target for radiation therapy in malignant glioma
| Project/Area Number |
22791170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Gunma University |
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Principal Investigator |
NODA Shinei 群馬大学, 医学部, 助教 (60396645)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 神経膠芽腫 / GRP78 / 放射線治療 |
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| Research Abstract |
Stable knockdown cell lines of GRP78 using lentiviral based sh-RNA transduction were generated in 3 glioblastoma (GBM) cell lines. GBM cell lines were exposed 6 Gy of gamma radiation and subsequently evaluated to determine the extent of double strand DNA damage using comet and gamma-H2Ax assay and the radiosensitivity using colony formation assay and MTS assay. Apoptosis was evaluated using flowcytometry and westernblot assay.
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