The sensitivity of thyroid cancer stem cells to radioiodine

2012 Fiscal Year Final Research Report

• Project/Area Number: 22791204
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Nagasaki University
• Principal Investigator: MATSUSE Michiko 長崎大学, 医歯薬学総合研究科, 助教 (30533905)
• Project Period (FY): 2010 – 2012
• Keywords: 放射線治療生物学

Research Abstract

The purpose of this study was to isolate thyroid cancer stem cells and examine whether the sensitivity of the cancer stem cells to radioiodine is different from non-cancer stem cells. First, we established an efficient screening system to evaluate markers for thyroid cancer stem cells. Using this system, we were able to identify candidate markers such as ALDH1 activity and CD326. However, they were not universal markers in all types of thyroid cancers. To characterize the sorted subpopulations, radiosensitivity of each fraction was evaluated. However, there was no association between these markers and radiosensitivity.

Research Products

• [Journal Article] Functional characterization of the novel BRAF complex mutation, BRAFV600delinsYM, identified in papillary thyroid carcinoma (2013)
  • Author(s): Matsuse M, Mitsutake N, Tanimura S, Ogi T, Nishihara E, Hirokawa M, Fuziwara CS, Saenko VA, Suzuki K, Miyauchi A, Yamashita S
  • Journal Title: Int J Cancer Volume: 132(3) Pages: 738-43
  • DOI: doi:10.1002/ijc.27709
  • Peer Reviewed
• [Journal Article] Identification of the first ATRIP-deficient patient and novel mutations in ATR define a clinical spectrum for ATR-ATRIP Seckel Syndrome (2012)
  • Author(s): Ogi T, Walker S, Stiff T, Hobson E, Limsirichaikul S, Carpenter G, Prescott K, Suri M, Byrd PJ, Matsuse M, Mitsutake N, Nakazawa Y, Vasudevan P, Barrow M, Stewart GS, Taylor AM, O'Driscoll M, Jeggo PA
  • Journal Title: PLoS Genet Volume: 8(11) Pages: e1002945
  • DOI: doi:10.1371/journal.pgen.1002945
  • Peer Reviewed
• [Journal Article] Imatinib enhances docetaxel-induced apoptosis through inhibition of nuclear factor-kB activation in anaplastic thyroid carcinoma cells (2012)
  • Author(s): Kim E, Matsuse M, Saenko V, Suzuki K, Ohtsuru A, Mitsutake N, Yamashita S
  • Journal Title: Thyroid Volume: 22(7) Pages: 717-24
  • DOI: doi:10.1089/thy.2011.0380
  • Peer Reviewed
• [Journal Article] Copy number alteration and uniparental disomy analysis categorizes Japanese papillary thyroid carcinomas into distinct groups (2012)
  • Author(s): Matsuse M, Sasaki K, Nishihara E, Minami S, Hayashida C, Kondo H, Suzuki K, Saenko V, Yoshiura K, Mitsutake N, Yamashita S
  • Journal Title: PLoS One Volume: 7(4) Pages: e36063
  • DOI: doi:10.1371/journal.pone.0036063
  • Peer Reviewed
• [Journal Article] Mutations in UVSSA cause UV-sensitive syndrome and impair RNA polymerase IIo processing in transcription-coupled nucleotide-excision repair (2012)
  • Author(s): Nakazawa Y, Sasaki K, Mitsutake N, Matsuse M, Shimada M, Nardo T, Takahashi Y, Ohyama K, Ito K, Mishima H, Nomura M, Kinoshita A, Ono S, Takenaka K, Masuyama R, Kudo T, Slor H, Utani A, Tateishi S, Yamashita S, Stefanini M, Lehmann AR, Yoshiura K, Ogi T
  • Journal Title: Nat Genet Volume: 44(5) Pages: 586-92
  • DOI: doi:10.1038/ng.2229
  • Peer Reviewed
• [Journal Article] miR-196a downregulation increases the expression of type I and III collagens in keloid fibroblasts (2012)
  • Author(s): Kashiyama K, Mitsutake N, Matsuse M, Ogi T, Saenko VA, Ujifuku K, Utani A, Hirano A, Yamashita S
  • Journal Title: J Invest Dermatol Volume: 132(6) Pages: 1597-604
  • DOI: doi:10.1038/jid.2012.22
  • Peer Reviewed
• [Journal Article] Absence of common activating mutations of the epidermal growth factor receptor gene in thyroid cancers from American and Japanese patients (2012)
  • Author(s): Ricarte-Filho JC, *Matsuse M, Lau C, Ryder M, Nishihara E, Ghossein RA, Ladanyi M, Yamashita S, Mitsutake N, Fagin JA
  • Journal Title: Int J Cancer Volume: 130(9) Pages: 2215-7
  • DOI: doi:10.1002/ijc.26267
  • Peer Reviewed
• [Journal Article] The FOXE1 and NKX2-1 loci are associated with susceptibility to papillary thyroid carcinoma in the Japanese population (2011)
  • Author(s): Matsuse M, Takahashi M, Mitsutake N, Nishihara E, Hirokawa M, Kawaguchi T, Rogounovitch T, Saenko V, Bychkov A, Suzuki K, Matsuo K, Tajima K, Miyauchi A, Yamada R, Matsuda F, Yamashita S
  • Journal Title: J Med Genet Volume: 48(9) Pages: 645-8
  • DOI: doi:10.1136/jmedgenet-2011-100063
  • Peer Reviewed
• [Journal Article] Dedifferentiation of human primary thyrocytes into multilineage progenitor cells without gene introduction (2011)
  • Author(s): Suzuki K, Mitsutake N, Saenko V, Suzuki M, Matsuse M, Ohtsuru A, Kumagai A, Uga T, Yano H, Nagayama Y, Yamashita S
  • Journal Title: PLoS One Volume: 6(4) Pages: e19354
  • DOI: doi:10.1371/journal.pone.0019354
  • Peer Reviewed
• [Journal Article] Induction of micronuclei in germinating onion seed root tip cells irradiated with high energy heavy ions (2010)
  • Author(s): Takatsuji T, Takayanagi H, Morishita K, Nojima K, Furusawa Y, Nakazawa Y, Matsuse M, Akamatsu S, Hirano N, Hirashima N, Hotokezaka S, Ijichi T, Kakimoto C, Kanemaru T, Koshitake M, Moriuchi A, Yamamoto K, Yoshikawa I
  • Journal Title: J Radiat Res Volume: 51(3) Pages: 315-23
  • URL: https://www.jstage.jst.go.jp/article/jrr/51/3/51_09028/_article
  • Peer Reviewed
• [Journal Article] miR-195, miR-455-3p and miR-10a(*) are implicated in acquired temozolomide resistance in glioblastoma multiforme cells (2010)
  • Author(s): Ujifuku K, Mitsutake N, Takakura S, Matsuse M, Saenko V, Suzuki K, Hayashi K, Matsuo T, Kamada K, Nagata I, Yamashita S
  • Journal Title: Cancer Lett Volume: 296(2) Pages: 241-8
  • DOI: doi:10.1016/j.canlet.2010.04.013
  • Peer Reviewed
• [Presentation] 甲状腺癌幹細胞マーカーの網羅的解析 (2012)
  • Author(s): 嶋村 美加
  • Organizer: 第55回日本甲状腺学会
  • Place of Presentation: 福岡
  • Year and Date: 20121129-1201
• [Presentation] FOXE1及びNKX2-1ローカスにおける遺伝子多型は日本人甲状腺乳頭癌に関連する遺伝的因子である (2011)
  • Author(s): 松瀬 美智子
  • Organizer: 第54回日本甲状腺学会
  • Place of Presentation: 大阪
  • Year and Date: 20111121-23
• [Presentation] 甲状腺乳頭癌症例で検出された新規BRAF変異(BRAF^)の機能解析 (2011)
  • Author(s): 松瀬 美智子
  • Organizer: 第54回日本甲状腺学会
  • Place of Presentation: 大阪
  • Year and Date: 20111121-23
• [Presentation] Imatinib, the selective tyrosine kinase inhibitor, enhances antitumor activity of docetaxel in anaplastic thyroid cancer cells (2010)
  • Author(s): Michiko Matsuse
  • Organizer: 14th International Thyroid Congress
  • Place of Presentation: Paris, France
  • Year and Date: 20100914-15