2011 Fiscal Year Final Research Report
Induction of tolerance to small intestine allografts by adoptive transfer of in vitro generating regulatory T-cells
| Project/Area Number |
22791262
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
ABE Yuta 東京医科大学, 医学部, 助教 (70327526)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 外科 / 免疫学 |
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| Research Abstract |
We demonstrate the in vitro generation of regulatory T cells(RA-Treg) in the presence of IL-2, TGF beta and all trans retinoic acid(RA) to acquire a gut-homing ability and more potent at suppressing T-cell activation in vitro. We also demonstrate that RA-Treg injected into Naive mice, intestinal ischemia/reperfusion injury mice, syngeneic intestine transplanted mice, allogeneic intestine transplanted mice respectively can reach recipient mesenteric nodes. More increased accumulation of RA-Treg was observed in I/R injury mice, syngeneic transplanted mice, allogeneic transplanted mice than that in naive mice demonstrating the role of I/R injury for accumulation Tregs in this model.
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