2011 Fiscal Year Final Research Report
Identification of tumor suppressive miRNAs induced by histone deacetylase inhibitor vorinostat in human breast cancer cells
| Project/Area Number |
22791263
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | HDAC / inhibitor / microRNA / breast cancer |
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| Research Abstract |
Vorinostat is a histone deacetylase inhibitor that effectively suppresses cancer-cell proliferation by inducing cell-cycle arrest and/or apoptosis. Emerging evidence suggests that aberrant expression of microRNAs(miRNAs), single-strand RNAs of 18-24 nt express endogenously, plays pivotal roles in cancer pathogenesis. The aim of the present study was to identify tumor suppressive miRNAs through comparison of miRNA expression profiles of MDA-MB-231 cells treated with/without vorinostat using PCR-based miRNA array system. We identified 18 miRNAs, which were significantly up-regulated after 24 h treatment with vorinostat. In addition, four up-regulated and three down-regulated miRNAs were also identified after 48 h treatment with vorinostat. Among these miRNAs, four of them were persistently up-regulated for up to 48 h after vorinostat treatment. These miRNAs may be useful as a molecular target for breast cancer therapy or a diagnosis marker.
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