2011 Fiscal Year Final Research Report
Research on gene copy number and resistance to EGFR-TKI therapy using silver in situ hybridization(SISH) technology for non-small-cell lung cancer
Project/Area Number |
22791323
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Thoracic surgery
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Research Institution | Tokyo Medical University |
Principal Investigator |
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Project Period (FY) |
2010 – 2011
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Keywords | 肺癌 / EGFR / EGFR-TKI / EGFR遺伝子変異 / 上皮成長因子受容体 / 分子標的治療 / 薬剤耐性 / IGF-1R / FISH / SISH |
Research Abstract |
An experimental SISH probe designed by Ventana Medical Systems(Tucson, AZ) was used to evaluate IGF1R gene copy number on 10 non-small-cell lung cancer(NSCLC) cell lines. The mean number of IGF1R gene copies/nuclei per cell line was 2.26(range : 1.12-7.56). The median copy number was 2.11.Squamous cell carcinoma had 7.56 copy number compared to non-SCC histologies. IGF1R gene copy number detected by SISH is higher in Squamous cell carcinoma cell line than in other cell line of NSCLC.
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[Journal Article] EGFR protein expression in non-small cell lung cancer predicts response to an EGFR tyrosine kinase inhibitor-a novel antibody for immunohistochemistry or AQUA technology2011
Author(s)
Mascaux C, Wynes MW, Kato Y, Tran C, Asuncion BR, Zhao JM, Gustavson M, Ranger-Moore J, Gaire F, Matsubayashi J, Nagao T, Yoshida K, Ohira T, Ikeda N, Hirsch FR
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Journal Title
Clin Cancer Res
Volume: 17(24)
Pages: 7796-807
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[Journal Article] Prognostic impact of number of resected and involved lymph nodes at complete resection on survival in non-small cell lung cancer2011
Author(s)
Saji H, Tsuboi M, Yoshida K, Kato Y, Nomura M, Matsubayashi J, Nagao T, Kakihana M, Usuda J, Kajiwara N, Ohira T, Ikeda N
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Journal Title
J Thorac Oncol
Volume: 6(11)
Pages: 1865-71
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[Journal Article] Novel epidermal growth factor receptor mutation-specific antibodies for non-small cell lung cancer : immunohistochemistry as a possible screening method for epidermal growth factor receptor mutations2010
Author(s)
Kato Y, Peled N, Wynes MW, Yoshida K, Pardo M, Mascaux C, Ohira T, Tsuboi M, Matsubayashi J, Nagao T, Ikeda N, Hirsch FR
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Journal Title
J Thorac Oncol
Volume: 5(10)
Pages: 1551-8
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[Presentation] The role of IGF-1R in EGFR TKI resistance in NSCLC using IHC and AQUA technology2011
Author(s)
Y. Kato, C. Mascaux, M. W. Wynes, B. Reyna Asuncion, C. Tran, K. Yoshida, J. Matsubayashi, E. Nakajima, T. Ohira, T. Nagao, K. Furukawa, N. Ikeda, F. R. Hirsch
Organizer
ASCO Annual Meeting
Year and Date
20110000