• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Effect of Cholesterol on Sphingosylphosphorylcholine-Rho-kinase -mediated Cerebral Vasospasm

Research Project

  • PDF
Project/Area Number 22791346
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionYamaguchi University

Principal Investigator

SHIRAO Satoshi  山口大学, 大学院・医学系研究科, 助教 (70448281)

Project Period (FY) 2010 – 2012
Keywordsラフト / コレステロール / スフィンゴ脂質 / 脳血管攣縮
Research Abstract

Lipid rafts are cholesterol-enriched membrane microdomains that influence signal transduction. In this study, hypercholesterolemia increased vascular smooth muscle (VSM) cholesterol (but not PC, a major component of nonraft membranes) and sphingosylphosphorylcholine(SPC)-ROK-mediated VSM contraction. Depletion of VSM cholesterol (but not PC) byβ-cyclodextrin(β-CD) inhibited SPC-induced contraction. Treatment with β-CD decreased the mean % filipin III staining intensity of Human brain VSM cells. Depletion of VSM cholesterol by β -CD also decreased the raft marker ganglioside GM1 count per unit membrane length and inhibited SPC-induced contraction. These results indicate that cholesterol potentiates SPC-ROK-mediated contractions of importance in cerebral vasospasm and are compatible with a role for lipid rafts in this process.

  • Research Products

    (5 results)

All 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Sphingosylphosphorylcholine-Rho-kinasemediated smooth muscle contraction in the rat basilar artery is regulated by the serum total cholesterol or vascular tissue cholesterol level: a mechanistic role for lipid rafts2011

    • Author(s)
      Satoshi Shirao, Hiroshi Yoneda, Mizuya Shinoyama, Yoshihiko Maeda, Hiroyasu Koizumi, Hideyuki Ishihara, Fumiaki Oka, Hirokazu Sadahiro, Sadahiro Nomura, Koji Kajiwara, and Michiyasu Suzuki
    • Journal Title

      Stroke

      Volume: 42(3) Pages: e134

    • Peer Reviewed
  • [Journal Article] Sphingosylphosphorylcholine が引き起こす脳血管攣縮におけるコレステロールの役割2010

    • Author(s)
      白尾敏之、米田 浩、篠山瑞也、藤井正美、野村貞宏、加藤祥一、鈴木倫保
    • Journal Title

      脳血管攣縮

      Volume: 26巻 Pages: 79-81

    • Peer Reviewed
  • [Presentation] コレステロールの役割について2011

    • Author(s)
      白尾敏之
    • Organizer
      日本脳神経外科学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜)
    • Year and Date
      2011-10-14
  • [Presentation] Sphingosylphosphorylcho lineRho-kinase-mediated smooth muscle contraction in the rat basilar artery is regulated by the serum total cholesterol or vascular tissue cholesterol level : amechanistic role for lipid rafts2011

    • Author(s)
      Satoshi Sirao
    • Organizer
      International Stroke Conference
    • Place of Presentation
      Los Angeles, California, USA
    • Year and Date
      2011-02-09
  • [Presentation] くも膜下出血後の脳血管攣縮に対する前向き実態調査 STROKE 20102010

    • Author(s)
      米田 浩、白尾敏之、石原秀行、末廣栄一、加藤祥一、鈴木倫保
    • Organizer
      第26回スパズム・シンポジウム
    • Place of Presentation
      岩手県民会館(岩手県盛岡)
    • Year and Date
      2010-04-15

URL: 

Published: 2014-08-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi