2011 Fiscal Year Final Research Report
Elucidation of the mechanism of cancer by methylation of histone and methylated genes in malignant brain tumor
| Project/Area Number |
22791350
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Saga University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | エピジェネティクス / メチル化 / 膠芽腫 / EHMT1 |
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| Research Abstract |
We examined the genetic and epigenetic aberration of EHMT1 gene in the several glioblastoma cell lines and clinical specimen. Genomic quantitative PCR was performed in glioblastioma cell lines, such as U87MG, T98G, U251MG, U118MG and A 172. None of all cell lines showed the loss of heterozygosity. We did not detect the mutation of the EHMT1 gene on genomic sequence. We also examine the expression levels of mRNA and protein of EHMT1 using quantitative RT-PCR and Western blotting method in glioblastoma cell lines and clinical specimen. The expression of mRNA and protein of EHMT1 did not decrease in those materials.
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