OASIS, a CREB/ATF-family transcription factor, modulate transcription of C6ST1 gene and chondroitin sulfation

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791353
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Nara Medical University
• Principal Investigator: OKUDA Hiroaki 奈良県立医科大学, 医学部, 助教 (40453162)
• Project Period (FY): 2010 – 2011
• Keywords: アストロサイト

Research Abstract

In the central nerve system, ER stress is implicated in a wide range of disorders including ischemic injury and neurodegenerative disorder. Both mRNA and protein levels of OASIS were elevated in the injured cortex and correlated closely with those of reactive astrocyte. Reactive astrocyte, which constitute a major cellular component of glial scar, enmesh the lesion site and produce anti-regenerative molecules such as chondroitin sulphate proteoglycans(CSPGs). We have analyzed the mRNA levels of the CS-glycosaminoglycan synthesizing enzymes using OASIS knockout mice. Expression of chondroitin 6-sulfotransferase 1(C6ST1) is increased in cortical stub injury in wild type mice, but is not in OASIS knock out mice. Furthermore, OASIS bound to the first intron region and promoted transcription of C6ST1.These results suggest that OASIS may be involved in CSPG production through the transcription of C6ST1 in astrocytes.