2011 Fiscal Year Final Research Report
The analysis of the molecular mechanism of 5ALA induced photodynamic diagnosis for malignant brain tumor and the challenge to application of photodynamic therapy for glioma stem cells
| Project/Area Number |
22791360
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
IKEDA Naokado 大阪医科大学, 医学部, 非常勤講師 (50434775)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 悪性脳腫瘍 / 神経膠芽腫 / 転移性脳腫瘍 / CPOX / 5-アミノレブリン酸 / プロトポルフィリンIX / 光線力学的診断 / 光線力学的治療 |
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| Research Abstract |
Little is known, however, about the detailed molecular mechanisms underlying 5-ALA-induced fluorescence.
We analyzed transcriptome profiles for the genes encoding enzymes, transporters, and a transcription factor involved in the porphyrin-biosynthesis pathway for surgical removed malignant brain tumors.
The high mRNA level of CPOX expression was significantly well correlated with the phenotype of strong 5-ALA induced fluorescence. These findings were further confirmed by immunohistochemical studies with a CPOX-specific antibody. It is concluded that induction of CPOX gene expression is one of the key molecular mechanisms underlying the 5-ALA-induced fluorescence of malignant brain tumors.
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