Establishment of the treatment and pathophysiology based on the susceptibility gene in lumbar disc disease.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22791365
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: University of Toyama
• Principal Investigator: SEKI Shoji 富山大学, 大学院・医学薬学研究部(医学), 助教 (00432112)
• Project Period (FY): 2010 – 2012
• Keywords: CILP / 腰椎椎間板 / トランスジェニックマウス

Research Abstract

Safranin O staining in intervertebral disc were significantly decreased in Nucleus pulposus tissues of Tg mice compared to controls. MRI analysis indicated obvious progression of degenerative intervertebral discs in Tg mice, which suggested reduction of the intensity of lumbar T2 weighted MR imaging. The cervical intervertebral disc degeneration and hernia models were established by introducing instability into cervical vertebrate of CILP Tg mice. The histological analysis suggested that progression of intervertebral disc degeneration and regulation of TGF-βsignaling. There may be a possibility that CILP enhance intervertebral disc degeneration.

Research Products

• [Journal Article] Cartilage Intermediate Layer Protein (CILP) Regulation in Intervertebral Discs: The Effect of Age (2012)
  • Author(s): Wang Z, Kim JH, Higashino K, Kim SS, Wang S, Seki S, Hutton WC, Yoon ST.
  • Journal Title: Degeneration, and BMP-2. Spine Volume: 37巻 Pages: E203-208
  • DOI: DOI:10.1097/BRS.0b013e31822dcf47.
  • Peer Reviewed
• [Journal Article] Isolation and characterization of human amniotic mesenchymal stem cells and their chondrogenic (2012)
  • Author(s): Nogami M, Tsuno H, Koike C, Okabe M, Yoshida T, Seki S, Matsui Y, Kimura T, Nikaido T.
  • Journal Title: differentiation.Transplantation Volume: 93巻 Pages: 1221-1229
  • DOI: DOI:10.1097/TP.0b013e3182529b76.
  • Peer Reviewed
• [Journal Article] Adamts5 遺伝子と椎間板変性 (2012)
  • Author(s): 関 庄二、淺沼由美子、舛田浩一、川口善治、淺沼邦洋、木村友厚
  • Journal Title: 整形・災害外科 Volume: 55巻 Pages: 118-119
• [Presentation] Mechanical stress loading of cervical intervertebral disc in CILP transgenic mice promotes intervertebral disc degeneration. (2012)
  • Author(s): Shoji Seki, Noriyuki Tsumaki, Hiraku Motomura, Yoshiharu Kawaguchi, Yoshito Matsui, Kayo Suzuki, Tomoatsu Kimura.
  • Organizer: ISSLS
  • Place of Presentation: アムステルダム(オランダ)
  • Year and Date: 20120528-0601
• [Presentation] Effect of a selective c-Fos/AP-1 inhibitor on the human nucleus pulposus cells. (2012)
  • Author(s): Yasuhito Yahara, Shoji Seki, Hiraku Motomura, Yoshito Matsui, Mami Higashimoto, Syunichi Shiozawa, Tomoatsu Kimura.
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2012-10-26
• [Presentation] c-Fos/AP-1阻害薬(T-5224)の軟骨および椎間板変性抑制効果 (2012)
  • Author(s): 関 庄二、元村 拓、塩沢俊一、木村友厚
  • Organizer: 応用薬理シンポジ ウム
  • Place of Presentation: ベルクラシック甲府(山梨県)
  • Year and Date: 2012-09-04
• [Presentation] ADAMTS5遺伝子 ノックダウンにおける椎間板変性抑制効果 (2010)
  • Author(s): 関 庄二, 阿部由美子,川口善治,浅沼邦洋, 舛田浩一,木村友厚
  • Organizer: 第83回日本整形外科学術集会
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 20100527-30
• [Presentation] CILP, cartilage intermediate layer protein, promotes lumbar disc degeneration. (2010)
  • Author(s): hoji Seki, Noriyuki Tsumaki, Yoshiharu Kawaguchi, * Yumiko Abe, Kayo Suzuki, Akiko Iwai, Takeshi Oya, Shiro Ikegawa, and Tomoatsu Kimura
  • Organizer: ORS
  • Place of Presentation: ニューオーリンズ(アメリカ)
  • Year and Date: 20100306-09