N-Acetylglucosamine 6-O-Sulfotransferase-1 is crucialenzyme for 5D4-reactive keratin sulfate biosynthesis which elicits expression after spinal cord injury.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791371
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Nagoya University
• Principal Investigator: ANDO Kei 名古屋大学, 医学部附属病院, 医員 (40566973)
• Project Period (FY): 2010 – 2011
• Keywords: GlcNAc6ST-1 / 脊髄損傷 / 脊髄腫瘍

Research Abstract

：5D4-reactive KSPGs were decrease as aging in wild type mice CNS, and were loss of expression in GlcNAc6ST-1-/- mice. On the other hand, BCD4-reactive KSPGs were increased as aging in wild type and GlcNAc6ST- 1-/- mice CNS. The KS enzyme b3GlcNAcT-7 was also significantly down-regulated not only GlcNAc6ST-1 in CNS of GlcNAc6ST-1-/- mice compared to that of wild type mice. 5D4-reactive KSPGs were decreased for cell lysates of GlcNAc6ST-1 siRNA transfected BV2, although BCD4-reactive KSPGs were not decreased. The enzyme b3GlcNAcT-7 was significantly downregulated not only GlcNAc6ST-1 same as in vivo study. The enzyme significantly down-regulated was only b3GlcNAcT-7. If the ablation of 5D4-reactive KS except for BCD4-reactive KS is possible, better functional recovery may be obtained. Ourdata suggested that GlcNAc6ST-1 was important enzyme for 5D4-reactive KSPGs biosynthesis, b3GlcNAcT-7 for both 5D4 and BCD4-reactive KSPGs biosynthesis. The data was supported with in vitro study used transfected microglia cell line. Interestingly, GlcNAc6ST-1 may regulate b3GclNAcT-7 by decrease of own expression. GlcNAc6ST-1 specific down-regulation treatment may be the key in CNS injury.

Research Products

• [Journal Article] Differentiation of Spinal Schwannomas and Myxopapillary Ependymomas: MR Imaging and Pathologic Features (2013)
  • Author(s): Kei Ando, Imagama S, Ito Z, Hirano K, Tauchi R, Muramoto A, Matsui H, Matsumoto T, Sakai Y, Matsuyama Y, Ishiguro N
  • Journal Title: J Spinal Disord Tech
  • Peer Reviewed
• [Journal Article] Examination of the influence of ossification of the anterior longitudinal ligament on symptom progression and surgical outcome of ossification of the thoracic ligamentum flavum: a multicenter study (2012)
  • Author(s): Kei Ando, Imagama S, Wakao N, Hirano K, Tauchi R, Muramoto A, Kato F, Yukawa Y, Kawakami N, Sato K, Matsubara Y, Kanemura T, Matsuyama Y, Ishiguro N
  • Journal Title: J Neurosurg Spine Volume: 16 Pages: 147-153
  • Peer Reviewed
• [Journal Article] N-acetylglucosamine 6-O-sulfotransferase-1-deficient mice show better functional recovery after spinal cord injury (2010)
  • Author(s): Ito Z, Sakamoto K, Imagama S, Matsuyama Y, Zhang H, Hirano K, Ando K, Yamashita T, Ishiguro N, Kadomatsu K
  • Journal Title: J Neurosci Volume: 30 Pages: 5937-47
  • Peer Reviewed
• [Presentation] N-Acetylglucosamine 6-O-Sulfotransferase-1 is crucial enzyme for 5D4-reactive keratin sulfate biosynthesis which elicits expression after spinal cord injury (2011)
  • Author(s): Kei Ando
  • Organizer: 2011 Annual meeting of Orthopedics Research society
  • Place of Presentation: long beach, USA
  • Year and Date: 2011-01-16