2011 Fiscal Year Final Research Report
L-serine starvation response in osteoclast precursors and its physiological significance in the bone marrow microenvironment
| Project/Area Number |
22791382
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
OGAWA Takuya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (30457147)
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| Project Period (FY) |
2010 – 2011
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| Keywords | アミノ酸 / セリン / 細胞・組織 / 発生・分化 / 発現制御 / シグナル伝達 / 骨・軟骨代謝学 / 破骨細胞 |
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| Research Abstract |
Osteoclasts play central roles in the development and remodeling of bone. In addition to the stimulation by RANKL, serine in the culture medium is necessary for the in vitro differentiation of osteoclasts from their precursors. We conducted a series of experiments to understand the regulatory mechanism of osteoclast differentiation by serine as well as its physiological significance, and found the activation of amino acid starvation response in the precursors by serine deprivation alone, low expression of serine biosynthetic enzymes, and importance of serine supplied by osteoblasts to osteoclasts in the bone marrow microenvironment.
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