The effect of sex hormone on canilaae metabolism

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791392
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Osaka City University
• Principal Investigator: MINODA Yukihide 大阪市立大学, 大学院・医学研究科・整形外科学, 講師 (90453133)
• Project Period (FY): 2009 – 2011
• Keywords: エストロゲン / 軟骨代謝 / ノックアウトマウス / cre-loxPシステム

Research Abstract

The effect of sex hormone on cartilage metabolism is unclear, and little has been reported about the effects of estrogen on the cartilage differentiation. Estrogen is essential for maintaining the balance between osteoblastic and osteoclastic activity during bone remodeling in adults. To investigate the mechanism by which estrogen enhances chondrogenic differentiation, in vitro experiments were carried out using ATDC5 cells(a mouse pre-chondrogenic cell line) and primary cultured chondrogenic cells of mouse rib. Alcian blue staining for cartilage nodules and real-time reverse-transcription polymerase chain reaction for the expression of genes encoding type II collagen. type X collagen and augrecan, were performed in cells treated with estrogen. The expression level of aggrecan and type II collagen in cells, a marker of chondrogenic differentiation, was consistently elevated by low dose of estrogen(10^<-12> M) and was suppressed by high dose(10^<-6> M). ATDC5 and primary cultured chondrogenic cells treated with certain concentration of estrogen have higher expression of Col II and aggrecan.

Research Products

• [Journal Article] Conventional or navigated total knee arthroplasty affects sagittal component alignment (2012)
  • Author(s): Sugama R, Minoda Y, Kobayashi A, Iwaki H, Ikebuchi M, Takaoka K, Nakamura H.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc
  • Peer Reviewed
• [Journal Article] Early biological fixation of porous implant coated with paste-retaining recombinant bone morphogenetic protein 2 (2012)
  • Author(s): Fukunaga K, Minoda Y, Iwakiri K, Iwaki H, Nakamura H, Takaoka K.
  • Journal Title: J Arthroplasty Volume: 27 Pages: 143-149
  • Peer Reviewed
• [Journal Article] Mid-term Mid-term clinical results of alumina medial pivot total knee arthroplasty. pivot total knee arthroplasty (2011)
  • Author(s): Iida T, Minoda Y, Kadoya Y, Matsui Y, Kobayashi A, Iwaki H, Ikebuchi M, Yoshida T, Nakamura H.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc.
  • Peer Reviewed
• [Journal Article] Sagittal alignment of the lower extremity while standing in female (2011)
  • Author(s): Sugama R, Minoda Y, Kobayashi A, lwaki H, Ikebuchi M, Hashimoto Y, Takaoka K, Nakamura H.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. Volume: 19 Pages: 74-9
  • Peer Reviewed
• [Journal Article] A cemented mobile-bearing total knee prosthesis prevents peri-prosthetic bone mineral density loss around the femoral component-a matched cohort study (2010)
  • Author(s): Minoda Y, Ikebuchi M, Kobayashi A, Iwaki H, Inori F, Nakamura H.
  • Journal Title: J Bone Joint Surg Br. Volume: 92 Pages: 794-8
  • Peer Reviewed
• [Journal Article] Comparison of the bone mineral density between porous tantalum and cemented tibial components after total knee arthroplasty. (2010)
  • Author(s): Minoda Y, Kobayashi A, Iwaki H, Ikebuchi M, Inori F, Takaoka K.
  • Journal Title: J Bone Joint Surg Am. Volume: 92 Pages: 700-6
  • Peer Reviewed
• [Journal Article] Analysis of sex and race and the size and shape of the distal femur using virtual surgery and archived computed tomography images. Barnes CL (2010)
  • Author(s): Iwaki H, Minoda Y.
  • Journal Title: J Surg Orthop Adv. Volume: 19(4) Pages: 200-8
  • Peer Reviewed
• [Journal Article] Are acetabular component alignment guides for total hip arthroplasty accurate? (2010)
  • Author(s): Minoda Y, Ohzono K, Aihara M, Umeda N, Tomita M, Hayakawa K.
  • Journal Title: J Arthroplasty. Volume: 25 Pages: 986-9
  • Peer Reviewed
• [Journal Article] The risk of notching the anterior femoral cortex with the use of navigation systems in total knee arthroplasty. (2010)
  • Author(s): Minoda Y, Kobayashi A, Iwaki H, Mitsuhiko I, Kadoya Y, Ohashi H, Takaoka K, Nakamura H.
  • Journal Title: Knee Surg Sports Traumatol Arthrosc. Volume: 18 Pages: 718-22
  • Peer Reviewed
• [Journal Article] In vivo polyethylene wear particle analysis of improved material and design in TKA (2009)
  • Author(s): Minoda Y, Kobayashi A, Iwaki H, Iwakiri K, Inori F, Sugama R, Ikebuchi M, Kadoya Y, Takaoka K.
  • Journal Title: J Bone Joint Surg Am. Volume: 91 Suppl 6 Pages: 67-73
  • Peer Reviewed
• [Journal Article] In vivo comparison of wear particles between highly crosslinked polyethylene and conventional polyethylene in the same design of total knee arthroplasties. (2009)
  • Author(s): Iwakiri K, Minoda Y, Kobayashi A, Sugama R, Iwaki H, Inori F, Hashimoto Y, Ohashi H, Ohta Y, Fukunaga K, Takaoka K.
  • Journal Title: J Biomed Mater Res B Appl Biomater. Volume: 91(2) Pages: 799-804
  • Peer Reviewed
• [Journal Article] Positive nasal culture of methicillin-resistant Staphylococcus aureus is a risk factor for surgical infection in orthopaedic surgeries. (2009)
  • Author(s): Yano K. Minoda Y, Sakawa A, Kuwano Y, Kondo K, Fukushima W, Tada K.
  • Journal Title: Acta Orthop. Volume: 80 Pages: 486-90
  • Peer Reviewed
• [Journal Article] High incidence of patellar clunk syndrome in a recently designed mobile bearing posterior stabilized total knee prosthesis. (2009)
  • Author(s): Fukunaga K, Kobayashi A, Minoda Y, Iwaki H, Hashimoto Y, Takaoka K.
  • Journal Title: J Bone Joint Surg Br. Volume: 91(4) Pages: 463-8
  • Peer Reviewed
• [Journal Article] Comparison between highly cross-linked and conventional polyethylene in total knee arthroplasty. (2009)
  • Author(s): Minoda Y, Aihara M, Sakawa A, Fukuoka S, Hayakawa K, Tomita M, Umeda N, Ohzono K.
  • Journal Title: Knee. Knee. Volume: 16(5) Pages: 348-51
  • Peer Reviewed
• [Journal Article] TKA sagittal alignment with navigation systems and conventional techniques vary only a few degrees. (2009)
  • Author(s): Minoda Y, Kobayashi A, Iwaki H, Ohashi H, Takaoka K.
  • Journal Title: Clin Orthop Relat Res. Volume: 467(4) Pages: 1000-6
  • Peer Reviewed
• [Journal Article] Range of Motion of Standard and High-Flexion Cruciate Retaining Total Knee Prostheses. (2009)
  • Author(s): Minoda Y, Aihara M, Sakawa A, Fukuoka S, Hayakawa K, Ohzono K.
  • Journal Title: J Arthroplasty. Volume: 24(5) Pages: 674-80
  • Peer Reviewed
• [Journal Article] Acetabular component orientation in intra-and postoperative positions in total hip arthroplasty. (2009)
  • Author(s): Hayakawa K, Minoda Y, Aihara M, Sakawa A, Ohzono K, Tada K.
  • Journal Title: Arch Orthop Trauma Surg. Volume: 129(9) Pages: 1151-6
  • Peer Reviewed
• [Presentation] Intra-Operative Assessment Of Mid-Flexion Instability In Total Knee Arthropalsty (2012)
  • Author(s): Minoda Y, Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, lwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
  • Organizer: Annual meeting of Orthopaedic Reserch Society(ORS)
  • Place of Presentation: San Francisco, CA, USA.
  • Year and Date: 2012-02-04
• [Presentation] The Extension Gap Becomes Tight and Valgus After Implantation of Mobile-bearing Total Knee Prosthesis. (2012)
  • Author(s): Minoda Y Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, Iwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
  • Organizer: Annual meeting of Orthopaedic Reserch Society(ORS)
  • Place of Presentation: San Francisco, CA, USA.
  • Year and Date: 2012-02-04
• [Presentation] Operative Technique Affected The Intra-operative Joint Kinematics Of Total Knee Arhtroplasty. (2012)
  • Author(s): Minoda Y. Nakagawa S, Kadoya Y, Kobayashi A, Tomari H, Kondo M, Iwaki H, Ikebuchi M, Yoshida T, Matsui Y, Sumitomo A, Sugama R, Mizokawa S, Higuma Y, Noguchi T, Hirakawa H, Nakamura H.
  • Organizer: Annual meeting of Orthopaedic Reserch Society(ORS)
  • Place of Presentation: San Francisco, CA, USA.
  • Year and Date: 2012-02-04
• [Presentation] In Vivo Wear Particles of Annealed Highly Cross-Linked Polyethylene in Total Hip Arthropalsty. (2012)
  • Author(s): Minoda Y Hata K, Iwaki H, Ikebuchi M, Yoshida T, Miyazaki N, Miyake Y, Nakamura H.
  • Organizer: Annual meeting of Orthopaedic Reserch Society(ORS)
  • Place of Presentation: San Francisco, CA, USA.
  • Year and Date: 2012-02-04
• [Presentation] Invited lecture ; Future expectation of TKA. (2011)
  • Author(s): Minoda Y, Kadoya Y, Kobayashi A, Iwaki H, Iwakiri K, lida T, Matusi Y, Ikebuchi M, Yoshida T, Nakamura H.
  • Organizer: 24th Annual meeting of the International Society for Technology in Arthroplasty.
  • Place of Presentation: Brues, Belgium.
  • Year and Date: 2011-09-20
• [Presentation] BMD Change Under Bisphosphonate Therapy-Comparison Between Mobile and Fixed Bearing TKA Prostheses-. (2011)
  • Author(s): Minoda Y, Hiroyoshi I, Ikebuchi M, Yoshida T, Nakamura H.
  • Organizer: 24th Annual meeting of the International Society for Technology in Arthroplasty.
  • Place of Presentation: Brues, Belgium.
  • Year and Date: 2011-09-20
• [Presentation] High incidence of anterior femoral cortex notching using navigation system in TKA. (2011)
  • Author(s): Minoda Y, Watanabe K, Iwaki H, Ikebuchi M, Yoshida T, lida T, Ikawa T, Hashimoto Y, Nakamura H.
  • Organizer: Annual meeting of American Association of Orthopaedic Surgeons.
  • Place of Presentation: San Diego, CA, USA.
  • Year and Date: 2011-02-15
• [Presentation] (2011)
  • Author(s): Minoda Y, Watanabe K, Iwaki H, Lkebuchi M, Yoshida T, Lida T, Ikawa T, Hashimoto Y, Nakamura H.
  • Organizer: Annual meeting of American Academy of Orthopaedic Surgeons(AAOS)
  • Place of Presentation: San Diego, CA, USA.
  • Year and Date: 2011-02-07
• [Presentation] Comparison of BMD around the Knee between Trabecular Metal and Cemented Tibial Component ; Four-year Follow-Up Study. (2011)
  • Author(s): Minoda Y, Kobayashi A, Ikebuchi M, Iwaki H, Inori F, Hashimoto Y, Yoshida T, lida T, Ikawa T, Yamasaki S, Nakamura H.
  • Organizer: Annual Meeting of Orthopaedic Research Society.
  • Place of Presentation: Long Beach, CA, USA.
  • Year and Date: 2011-01-13
• [Presentation] Flexion Gap Preparation Opens Both the Extension and Flexion Gap during Total Knee Arthroplasty. (2011)
  • Author(s): Minoda Y, Iwaki H, Ikebuchi M, Yoshida T, Iida T, Ikawa T, Yamasaki S, Hirokai N.
  • Organizer: Annual Meeting of Orthopaedic Research Society.
  • Place of Presentation: Long Beach, CA, LISA.
  • Year and Date: 2011-01-13
• [Presentation] A Cemented Mobile-Bearing Total Knee Arthroplasty Prevents Periprosthetic Loss of Bone Mineral Density around the Femoral Component ; Four-year Follow-Up Study. (2011)
  • Author(s): Minoda Y. Kobayashi A, M, Iwaki H. Inori F. Hashimoto Y. Yoshida T, Iida T, Ikawa T, Yamasaki S, Nakamura H.
  • Organizer: Annual Meeting of Orthopaedic Research Society.
  • Place of Presentation: Long Beach, CA, USA.
  • Year and Date: 2011-01-13