2011 Fiscal Year Final Research Report
Efficient derivation of neural stem/progenitor cells from common marmoset embryonic stem cells for preclinical study of spinal cord injury.
Project/Area Number |
22791404
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Orthopaedic surgery
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Research Institution | Keio University |
Principal Investigator |
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Project Period (FY) |
2010 – 2011
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Keywords | 脊椎脊髄病学 |
Research Abstract |
We established in vitro culture method to efficiently derive multipotent neural stem/progenitor cells(NS/PCs) from non-human primate, common marmoset embryonic stem cells(ESCs). These marmoset ESC-derived neurospheres could be passaged repeatedly and sequentially gave rise to neurons and glial cells, including both astrocytes and oligodendrocytes. Importantly, marmoset ESC-derived NS/PCs could generate functional neurons, and showed closer expression profiles to fetal brain-derived neurosphres by repeated passages. Thus, this culture system is applicable to derive donor cells for allograft transplantation into marmoset models.
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[Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells2011
Author(s)
Hiroko Shimada, Yohei Okada, Hayao Ebise, Shin-ichi Ota, Ikuo Tomioka, Erika Sasaki, Masaya Nakamura, Hideyuki Okano
Organizer
Neuroscience 2011, SfN's 41st annual meeting
Place of Presentation
The Walter E. Washington Convention Center, Washington, DC, U. S. A.
Year and Date
20111112-16
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