2011 Fiscal Year Final Research Report
In vivo analysis of the interaction between RANK and RANKL by using innovative fluorescent probes
| Project/Area Number |
22791409
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Ehime University (2011)
Japanese Foundation For Cancer Research (2010) |
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Principal Investigator |
HIKITA Atsuhiko 愛媛大学, 大学院・医学系研究科, 准教授 (60443397)
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| Project Period (FY) |
2010 – 2011
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| Keywords | RANKL / RANK / 破骨細胞 / インビボ蛍光イメージング / BiFC |
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| Research Abstract |
By utilizing a fluorescence detection system by binding of complementary parts of a fluorescent protein(BiFC), the genes needed for the detection of the interaction of RANK and RANKL, proteins necessary for the formation of osteoclasts whoch have a central role in bone resorption, were constructed. In a preliminary experiment in which these two genes were introduced into cells, fluorescence was detected under the condition when RANK and RANKL could bind together.
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