2011 Fiscal Year Final Research Report

The study of new therapeutic target for intractable pain associated with acute pancreatitis

Research Project

Project/Area Number	22791421
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Anesthesiology/Resuscitation studies
Research Institution	University of Toyama
Principal Investigator	OISHI Mioko 富山大学, 大学病院, 助教 (10536733)
Project Period (FY)	2010 – 2011
Keywords	疼痛管理学
Research Abstract	First, we found that the mRNA levels of interleukin-1b and monocyte chemotactic protein(MCP)-1 were significantly increased in the pancreas of caerulein-treated mice. The sensitivity of abdominal organs was enhanced in caerulein-injected mice, suggesting that caerulein caused pancreatic hyperalgesia. Moreover, repeated treatment with caerulein resulted in cutaneous tactile allodynia of the upper abdominal region indicating that caerulein-treated mice exhibited referred pain. Under this condition, the mRNA level of cox-2 was significantly increased in the spinal cord. The mRNA levels of bradykinin B1 receptor(BKB1R) and bradykinin B2 receptor(BKB2R) were significantly increased in the dorsal root ganglion. Finally, we found that intrathecal injection of celecoxib(selective cox-2 inhibitor) attenuated the acute pancreatitis pain-like state in caerulein-treated mice. These findings suggest that the upregulation of cox-2 in the spinal code and BK receptors in the DRG may, at least in part, contribute to the development of the acute pancreatitis pain-like state in mice.