Cellular and molecular biological analysis of the impact of drugs used during periorerative period on cellular oxygen metabolism

2011 Fiscal Year Final Research Report

• Project/Area Number: 22791428
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Kyoto University
• Principal Investigator: TAKABUCHI Satoshi 京都大学, 医学研究科, 研究員 (90402841)
• Project Period (FY): 2010 – 2011
• Keywords: 薬理学 / 麻酔薬 / 低酸素応答 / 細胞生物学 / 分子生物学

Research Abstract

We demonstrated that Glucose-stimulated insulin secretion(GSIS) elicited by 400 mg/dL glucose was inhibited by treatment with isoflurane and sevoflurane. Taken together experimental evidence that glibenclamide-stimulated insulin secretion was not affected by either isoflurane or sevoflurane and that treatment with isoflurane or sevoflurane significantly suppress glucose-induced intracellular ATP elevation, it is suggested that isoflurane and sevoflurane affect mitochondria ETC and suppresses GSIS. Our finding may delineate the molecular mechanism behind the glucose tolerance established by volatile anesthetics.

Research Products

• [Journal Article] Hydrogen sulfide inhibits hypoxia-but not anoxia-induced hypoxia-inducible factor 1activation in a von hippel-lindau-and mitochondria-dependent manner (2011)
  • Author(s): Kai, S., Tanaka, T., Daijo, H., Harada, H., Kishimoto, S., Suzuki, K., Takabuchi, S., Takenaga, K., Fukuda, K., and Hirota, K.
  • Journal Title: Antioxidants & redox signaling Volume: 16 Pages: 203-216
  • DOI: DOI:10.1089/ars.2011.3882
  • Peer Reviewed
• [Journal Article] Fentanyl activates hypoxia-inducible factor 1 in neuronal SH-SY5Y cells and mice undernon-hypoxic conditions in a mu-opioidreceptor-dependent manner (2011)
  • Author(s): Daijo, H., Kai, S., Tanaka, T., Wakamatsu, T., Kishimoto, S., Suzuki, K., Harada, H., Takabuchi, S., Adachi, T., Fukuda, K., Hirota, K.
  • Journal Title: European journal of pharmacology Volume: 667 Pages: 144-152
  • DOI: DOI:10.1016/j.ejphar.2011.06.014
  • Peer Reviewed
• [Journal Article] The intravenous anesthetic propofol inhibits lipopolysaccharide-induced hypoxia-inducible factor 1 activation and suppresses the glucose metabolism in macrophages
  • Author(s): Tanaka, T., Takabuchi, S., Nishi, K., Oda, S., Wakamatsu, T., Daijo, H., Fukuda, K., and Hirota, K.
  • Journal Title: J Anesth Volume: 24 Pages: 56-60
  • DOI: DOI:10.1007/s00540-009-0829-1
  • Peer Reviewed
• [Presentation] Differential role of prostaglandin E-type receptors in activation of hypoxia-inducible factor1 by prostaglandin E1 in vascular-derived cells under non-hypoxic conditions (2011)
  • Author(s): Kengo Suzuki, Satoshi Takabuchi, Hiroki Daijo, Shinichi Kai, Tomonori Matsuyama, Shin Kurosawa, Kazuhiko Fukuda, Kiichi Hirota
  • Organizer: 2011Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Chicago
  • Year and Date: 2011-10-16
• [Presentation] Hydrogen sulfide inhibits hypoxia-induced HIF-1 activation in a mitochondria-dependent manner (2011)
  • Author(s): Shinichi Kai, Tomoharu Tanaka, Hiroki Daijo, Shun Kishimoto, Kengo Suzuki, Satoshi Takabuchi, Takuhiko Wakamatsu, Kazuhiko Fukuda, Kiichi Hirota
  • Organizer: 2011 Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Chicago
  • Year and Date: 2011-10-16