2012 Fiscal Year Final Research Report
Gene therapy for neuropathic pain applying SNP pace maker ion-channel (HCN channel) transduction to the spinal cord and dorsal root ganglion
| Project/Area Number |
22791433
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Okayama University |
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Principal Investigator |
KAKU Ryuji 岡山大学, 大学院・医歯薬学総合研究科, 助教 (50444659)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 遺伝子治療 / 神経因性疼痛 / 慢性痛 / ペースメーカーチャンネル |
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| Research Abstract |
HCN ion channels also know as a pace-maker channel play an important role on regulating spontaneous firing of central nerve systems at various neuropathic pain state. In this study, we investigated about the mechanisms of the HCN ion channel expression change to the nerve system. And we also investigated that the possibility of dominant-negative HCN ion channel as the source for the gene therapy to chronic neuropathic pain. We found that increase of cyclic AMP concentration decreased the glycosylated HCN ion channels on the plasma membrane. Non-glycosylated (SNP) HCN channels could reach to the plasma membrane of xenopus oocyte, but it did not pass the current. These findings suggested that the modulation of HCN channel expression on the spinal cord might be useful as the new treatment of chronic neuropathic pain.
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