2012 Fiscal Year Final Research Report
Analysis of analgesic action of remifentanil through dimerized mu-delta opioid receptors
| Project/Area Number |
22791441
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Research Collaborator |
UEZONO Yasuhito 独立行政法人国立がん研究センター, 研究所, 分野長 (20213340)
SUDO Yuka 独立行政法人国立がん研究センター, 研究所, 研究支援者 (70646695)
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| Project Period (FY) |
2010 – 2012
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| Keywords | オピオイド受容体 / 二量体 / レミフェンタニル / 急性耐性 |
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| Research Abstract |
Baby hamster kidney cells transfected with Venus-fused mu opioid receptors and Cerulean-fused delta opioid receptors were treated with remifentanil. To investigate the remifentanil-induced internalization and resensitization of the heterodimers, cells were fixed and examined by confocal microscopy after treatment of remifentanil. Fluorescence resonance energy transfer analysis was performed to confirm the heterodimerization of mu opioid receptors and delta opioid receptors. Remifentanil caused internalization of mu-delta heterodimers in a dose-dependent manner. Compared to our previous study using baby hamster kidney cells transfected with mu opioid receptors alone, baby hamster kidney cells transfected with mu opioid receptors and delta opioid receptors were more efficiently returned to plasma membrane after the remifentanil-induced internalization.
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